摘要：随着中药现代化的发展，利用微生物对中药进行发酵将有望成为中药使用领域的一大有力工具，而真菌在微生物中具有多重优势，是发酵中药的主要功能菌。本论文利用药用真菌灵芝En2和裂褶菌DS1对六种糖苷型化合物芦丁、黄芩素、苦杏仁苷、橙皮苷和槲皮素进行生物转化，检测转化结果并对其进行分析探讨。结果发现，菌株En2对于芦丁、黄芩素和槲皮素有明显的转化效果，菌株DS1对于黄岑素、甘草酸和槲皮素有明显的转化效果。对苦杏仁苷和橙皮苷，两株菌株其转化效果均不明显。对于芦丁、黄芩素和槲皮素，过程中投料两种真菌的转化效果更明显，对于甘草酸则在接种前投料菌株DS1的转化效果更明显。展望，可以将样品利用HPLC进行进一步检测，分析其含量，同时可将可能的新物质进行分离提纯，对其进行理化性质及药理作用检测，以得到新型药物。57504

毕业论文关键词：灵芝；裂褶菌；糖苷型化合物；紫外分光光度；生物转化

 Abstract：With the development of modernization of traditional Chinese medicine, the use of microbial fermentation of Chinese medicine is expected to become a powerful tool in the field of traditional Chinese medicine using.The fungus has many special, such as multiple secondary metabolites, more variety, and relatively simple culture conditions, etc., so is the main function of bacterial fermentation medicineThe experimental use of medicinal fungi(Ganoderma En2 and Schizophyllum DS1) on six kinds of glycoside compounds(Rutin, baicalein, laetrile, hesperidin and quercetin) for biotransformation, the researchers detected and analyzed the conversion resultsThrough the analysis we can conclude that, En2 significant transformation effect for rutin, quercetin and baicalein; DS1 significant transformation effect for Scutellaria hormone, licorice acid and quercetin. For laetrile and hesperidin, DS1 significant transformation ineffect. For rutin, baicalein and quercetin, the process of feeding the two fungi transforming effect is more obvious for the inoculation of glycyrrhizin in feed conversion effect before DS1 more obvious.Prospect, we can use the sample for further testing, and the new material may be separated and purified, the physicochemical properties of the samples and detection of pharmacology of the samples, we can get new drugs.

Keywords：Ganoderma Lucidum, Schizophyllum commune, glucoside compound, ultraviolet spectrophotometry, biotransformation

目  录

1. 前 言 5

1.1 生物转化 5

1.2 药用真菌简介 5

1.3 几种糖苷型化合物简介 6

1.4本论文技术路线 7

2. 材料与方法 8

2.1 材料 8

2.1.1 药品与试剂 8

2.1.2 菌种 8

2.1.3 仪器 8

2.2 方法 8

2.2.1 菌种的培养 8

2.2.2 药品的生物转化及取样 8

2.2.3 样品UV图谱扫描 9

3. 结果与讨论 10

3.1 芦丁生物转化结果及分析 10

3.1.1 接种前投料结果分析 10

3.1.2 过程中投料结果分析 11

3.1.3 接种前投料与过程中投料对比分析 12

3.2 黄芩素转化结果分析探究