摘要在哺乳动物，听觉感觉器官（螺旋器）的形成是限定在耳蜗的特定区域内的。然而，在耳蜗侧面分散区域中限制性感觉器官形成的分子机制尚不清楚，也不知耳蜗的其他区域是是否有形成螺旋器的能力。我们确定在限定区域内唯一的核酸蛋白——LMO4是耳蜗内听觉感觉器官形成的负调节蛋白。核酸蛋白LMO系列LOM1—LOM4在发育的内耳中在不同区域表达，并且它们在细胞的生长和分化中有数不清的作用，与螺旋器之间有紧密联系，故LMO1、LMO4与听力障碍有密切的关联。老鼠的Lom4的失活会导致异常的螺旋器位于侧耳蜗。这种螺旋器具有固有器官的特点，它包括内外毛细胞、支持细胞和其他非感觉特殊的细胞类型。这些数据表明这个在侧耳蜗管由lmo4调控的这片新颖的感觉的区域或许可应用于治疗操作。文章主要是想通过对小鼠LMO1、LMO4的一系列的研究来观察其对螺旋器的影响60557

Abstract In mammals, formation of the auditory sensory organ (the organ of Corti) is limited to a specific region of the cochlea. However, the molecular mechanism limiting sensory formation to this discrete region in the ventral cochlear duct is not clear, and we do not know whether the other regions of the cochlea are the ability to form the organ of Corti. Here we identify LMO4 that is a only nuclear protein in a limited area is negative regulator of auditory sensory organ formation in the cochlea. the LMO family of nuclear proteinsLMO1 and LMO4 are expressed in the developing inner ear in temporally and spatially distinct regions  and they have numerous roles in cell growth and differentiation。there are close links between the organ of Corti。 so LMO1, LMO4 with hearing impairment are closely related. If Lom4 of the mice lost activity, the organ of Corti in lateral cochlear would become abnormality. The ectopic organ of Corti (eOC) has a characteristic of native organs, which includes inner and outer hair cells, support cells and other nonsensory specialized cell types. These data can indicate a novel sensory competent region in the lateral cochlear duct that is regulated by LMO4 and may be amenable to therapeutic manipulation. The paper observers the effect of Lom1 and Lom4 on the organ of Corti .

毕业论文关键词：LMO4；LMO1；耳蜗；螺旋器；毛细胞

Key words: LMO4; LMO1; cochlea; the organ of Corti; hair cell

 目录 3

引言 4

1 材料与方法 5

1.1  实验材料 5

1.2实验方法 5

1.1.1基因型的鉴定 6

1.2.2组织准备 6

1.2.3荧光抗体标记 7

2  结果与分析 7

2.1 LMO1、LOM4基因敲除的方法 7

2.2对小鼠基因型的鉴定并进行筛选 8

2.3 LOM1、LOM4基因敲除后对小鼠内耳螺旋器的影响 9

2.3.1LOM1基因敲除后对小鼠内耳螺旋器的影响 10

2.3.2LOM4基因敲除后对小鼠内耳螺旋器的影响 10

2.3.3LOM1和LOM4基因敲除后对小鼠内耳螺旋器的影响 10

3  讨论 11

3.1 研究意义 11

3.2 Lom1和Lom4基因对小鼠内耳形态变化的影响 12

3.3 Lom1和Lom4基因的研究