摘要躁狂症是较为常见的精神疾病之一，其诊断标准不完善和治疗效果不明显。本实验以狂躁症小鼠为模型研究其发病机制，为狂躁症开发有效治疗提供理论依据。该模型小鼠是基因工程小鼠（TRE—ErbB2）其表型可以遗传。对其做行为学实验和病理机制的研究，发现Waltzer小鼠与正常小鼠在行为学存在比较大的差异，主要表现在waltzer小鼠表现为对刺激反应过度强烈，受到刺激自发快速地水平旋转，运动活跃以兴奋的状态存在。其抑郁倾向、运动作协调性和空间记忆能力比正常小鼠差。waltzer小鼠的焦虑状态比正常小鼠弱，运动能力和抗疲劳能力与正常小鼠差异不大。通过蛋白质印迹和免疫荧光技术对其病理机制研究，从目前的实验结果看总体差异不大。小鼠脑区整体形状轮廓（HE染色结果)上看该小鼠的脑室比正常小鼠要大。该小鼠模型具有典型的精神病症，其研究结果具有潜在的医学转换价值。62390

Abstract Psychomania is one of the commonest mental illnesses, which is lack of complete diagnostic criteria and obvious treatment effect. In order to study its mechanisms and provide theoretical basis for developing effective treatment for mania, this experiment takes hyperactive mouse as model. The mice were genetically engineered mice (TRE-ErbB2) whose phenotype can be genetic. Through the research of behavioristics pathological mechanisms, it is found that Waltzer mice have big differences with normal mice. These differences are mainly showing in the over-responses to the stimulation, spontaneous rapid horizontal rotation under stimulation, and movement active in the excited state on the Waltzer are worse than the normal mice. Waltzer anxiety in mice is weaker than normal mice, and exercise capacity and resistance to fatigue have little difference with normal mice. By Western blot and immunofluorescence study on pathological mechanism, the present experimental results show little overall difference. Mouse brain regions the overall shape (HE staining) have larger ventricles in mice than in the normal mice. The mouse model is typical of mental illness, and its research findings have potential medical conversion value.

毕业论文关键词： 狂躁症小鼠； 行为学实验； 病理机制   

Keyword:  Hyperactive mouse; Behavioral science experiments; Pathological mechanism

引言 4

1.材料与方法 5

1.1实验材料 5

1.1.1实验小鼠 5

1.1.2主要试剂和仪器 5

1.2实验方法 7

1.2.1行为研究（确立诊断狂躁指标） 7

1.2.1.1强迫游泳 7

1.2.1.2旷场实验 8

1.2.1.3水迷宫 8

1.2.2病理研究实验 8

1.2.2.1蛋白质印迹（Western blot）初步探讨病理机制。 8

2.结果与分析 10

2.1行为学实验数据 10

2.1.1强迫游泳 11

2.1.2旷场实验 11

2.1.3水迷宫实验 12

2.1.4转棒实验 13

2.2病理分析 13

2.2.1HE染色 13

2.2.2WB实验 13

2.2.3免疫荧光