摘要：近年来，随着环境污染日渐严重、气候变暖、能源短缺问题日显突出，在木质纤维资源生物炼制燃料乙醇和生物基化学品时，纤维素酶就起到关键作用。而用于生产纤维素酶的菌种主要是木霉属、曲霉属和青霉属。其中研究的比较多的菌株是里氏木霉。本实验以里氏木霉为研究对象,通过单因素实验研究培养基不同种类及浓度的单一氮源和不同比例的复合氮源对产纤维素酶的影响，单一氮源选用硫酸铵、尿素和硝酸钠，复合氮源选用不同比例的硫酸铵和尿素。实验结果表明单一氮源以0.20%的尿素酶活最高，酶活力达到0.23U，复合氮源以5:5硫酸铵比尿素的酶活力最高，酶活力达到0.28U，效果优于单一氮源。65514

关键词：里氏木霉，纤维素酶，氮源，滤纸酶活FPA

Abstract: In recent years, with increasingly serious environmental pollution, global warming, energy shortages become increasingly prominent. Cellulase plays a key role in the production of biethanol and bio-based chemicals from wood fiber resources. The microbes used in the production of cellulase are mainly Trichoderma, Aspergillus and Penicillin, in which Trichoderma reesei is widely studied. This thesis studied effect of nitrogen source in the production of cellulase by T. reesei through single factor experiments and different ratios of complex nitrogen source for cellulase production. The single nitrogen source chose ammonium sulfate, urea, sodium nitrate; complex nitrogen source used different proportions of ammonium sulfate and urea. The results showed that 2.0% of the urease gives the highest cellulase activity as single nitrogen source, with cellulase activity reaches 0.23U; 5: 5 ratio of ammonium sulfate and urea gives the highest cellulase activity as complex nitrogen source, with cellulase activity reaches 0.28U, better than single nitrogen source.

Keywords：Trichoderma reesei, Cellulase, Nitrogen source, Filter paper enzyme activity FPA

目录

摘要 3

1 前言 6

1.1 纤维素酶的概述 6

1.1.1 纤维素酶的分类及降解机制 7

1.1.2 纤维素酶的结构 7

1.1.3 纤维素酶的研究动态 7

1.2 里氏木霉的概述 7

1.2.1 里氏木霉产纤维素酶的概况 7

1.2.2 里氏木霉的特性 8

2 材料与方法 8

2.1 材料 8

2.1.1 菌株 8

2.1.2 主要仪器及设备 8

2.1.3 试剂配制 8

2.1.4 培养基配制 9

2.2 方法 9

2.2.1 里氏木霉的活化培养 9

2.2.2 纤维素酶制备 9

2.2.3 滤纸酶活测定 10

2.2.3.1 制备葡萄糖标准曲线 10

2.2.3.2 滤纸酶活的测定 10

2.2.3.3 酶活计算方法 10

3 结果与分析 11

3.1 数据处理 11

3.1.1 葡萄糖标准曲线 11

3.1.2 单一氮源