摘要本次研究课题通过从连云港附近废弃工厂污泥中采样，采用富集培养和直接涂布的方法以获得一种高效降解均三嗪类除草剂的菌株XR-D。该菌株在LB固体培养基上菌落形态为圆形，乳白色，表面光滑，凸状隆起，边缘整齐，分布较为密集，革兰氏阴性，在电子显微镜下为杆状菌，无鞭毛。进行16srRNA基因测序，构建系统发育树，以及形态学和生理生化分析，初步鉴定该菌株XR-D为鞘脂菌属（Sphingobium sp.）。利用分光光度计探究该菌株的最适生长PH为9，氯化钠浓度7%，通气量大小为100ml，同时对该菌株的碳素化合物的利用情况进行分析，发现该菌株的最佳碳源为葡萄糖，可以利用纤维二糖，乳糖，葡萄糖，果糖，山梨醇，维生素B1，不能利用甘露醇，蔗糖。 69931

该论文有图9幅，表2个，参考文献10篇。

毕业论文关键词：均三嗪  鞘脂菌属  降解菌  鉴定  生理生化分析

Isolation of Triazine herbicides degrading bacterium and its study of growth character

Abstract

The research by sampling the sludge from a nearby abandoned factory Lianyungang, using enrichment culture and direct coating method to obtain a degrading triazine herbicides strain XR-D. The strain on LB solid medium colony morphology round, white, smooth surface, the convex bulge, neat edges, are densely distributed, gram-negative, rod-shaped under the electron microscope bacteria without flagella. Conduct 16s rRNA gene sequencing, phylogenetic tree, as well as morphological, physiological and biochemical analysis, preliminary identification of the strain XR-D was identified as Sphingobium sp. Using a spectrophotometer inquiry optimum growth of the strain to PH 9, 7% sodium chloride concentration, the size of ventilation 100ml, while the utilization of carbon compounds strains were analyzed and found that the best carbon source strain of 1g / L glucose, you can utilize cellobiose, lactose, glucose, fructose, sorbitol, vitamin B1, cannot utilize mannitol, sucrose.

Key words: Triazine  Sphingobium  sp.  strain identification  Physiological and Biochemical Analysis

目  录

摘要Ⅰ

Abstract-Ⅱ

目录Ⅲ

图清单-Ⅳ

表清单-Ⅳ

1 绪论-1

1.1生物降解的研究现状与方法 -1

1.2本章小结-1

2 材料与方法-3

2.1土壤来源 3

2.2 培养基3

2.3 降解菌的富集培养与分离3

2.4 降解菌的形态特征观察3

2.5 降解菌的最适生长条件的测定3

2.6 降解菌的生理生化测定4

2.6 降解菌的16srRNA基因序列的测定5

3 结果与分析-5

3.1 降解菌的形态特征与分子水平鉴定5

3.2 降解菌的生理生化特性8

4 讨论11

参考文献12

附录113

附录215

附录319

致谢21

图清单

图序号 图名称 页码

图3-1 XR-D在LB固体培养基上的菌落形态

6

图3-2 菌株16srRNA基因扩增产物琼脂糖凝胶电泳图 7

图3-3 菌株XR-D的系统发育树

7

图3-4 XR-D菌株电镜照片

8

图3-5 菌株革兰氏染色图片 9

图3-6 不同盐浓度对菌株生长的影响 9

图3-7