摘要：以外切葡聚糖酶重组马克斯克鲁维酵母为对象，研究外切葡聚糖酶重组马克斯克鲁维酵母产酶的最佳培养基成分及条件。先确定最佳接种量、发酵培养基组分的最佳碳源、最佳碳源浓度、最佳氮源、最佳氮源浓度、最佳pH和最佳温度。等确定好最佳的条件后利用纤维素发酵生产乙醇。实验结果表明：在接种量以2%的量，以3%乳糖作为碳源，1%硫酸铵作为氮源，并且在pH为7.5和温度为121℃的条件下培养，发酵产生的酶活最大。通过优化实验，同时接种量以外切葡聚糖酶重组马克斯克鲁维酵母接种的种子液1.6mL和外切葡聚糖酶重组马克斯克鲁维酵母接种的种子液0.4mL，可以提高了马克斯克鲁维酵母的产酶活力，从而生产了更多的乙醇。 70569

毕业论文关键词：外切葡聚糖酶重组马克斯克鲁维酵母；培养条件的优化；生产乙醇 

Abstract: Outside cut recombinant dextranase Kluyveromyces marxianus as object, research cutting enzyme producing recombinant dextranase Kluyveromyces marxianus optimum culture medium compositions and conditions. The best carbon source, the best carbon source, the best carbon source, the best nitrogen source, the best nitrogen source, the best pH and the best temperature were determined. And so on to determine the best conditions for the use of cellulose fermentation to produce ethanol. The experimental results showed that the amount of 3G was 2%, and 1g was used as the carbon source, ammonium sulfate was used as nitrogen source, and the enzyme activity was maximum at pH 7.5 and 121. By optimizing the experiment, also inoculation volume outside the cut glucanase recombinant Kluyveromyces marxianus yeast seed liquid 1.6ml and glucanase recombinant Kluyveromyces marxianus yeast seed liquid 0.4ml cut, can improve the Kluyveromyces marxianus of enzyme activity, and produced more ethanol.

Keywords：Exoglucanases Marx recombinant Kluyveromyces; Optimization of cultivation conditions; roduction of ethanol  

目录

1 前言 7

1.1 纤维素酶的概述 7

1.2 马克斯克鲁维酵母的概述 7

2 材料与方法 7

2.1材料 8

2.1.1 菌株 8

2.1.2 主要仪器及设备 8

2.2 主要试剂 8

2.3 培养基 9

2.3.1 种子培养基（w/v） 9

2.3.2 发酵培养基（w/v） 9

2.4 纤维素酶酶活测定 9

2.4.1葡萄糖标准曲线的制作 9

2.4.2纤维素酶酶活测定 10

2.5 试验方法 10

2.5.1种子液制备 10

2.5.2摇瓶培养 10

2.6 摇瓶培养基的条件 10

2.6.1接种量大小对摇瓶培养产酶的影响 10

2.6.2不同碳源对摇瓶培养产酶的影响 10

2.6.3不同碳源浓度对摇瓶培养产酶的影响 10

2.6.4不同氮源对摇瓶培养产酶的影响 10

2.6.5不同氮源浓度对摇瓶培养产酶的影响 10

2.6.6初始酸碱性对摇瓶培养产酶的影响