小麦超氧化物歧化酶的生物信息学分析
关键词：小麦；超氧化物歧化酶；生物信息学
The Bioinformatics Analysis of Superoxide
Dismutase (SOD) of Wheat
Abstract: Superoxide dismutase (SOD for short) is a kind of protein family containing metallic element which is widely exists in the prokaryotic and eukaryotic organisms. It plays an important role in organisms, such as anti-aging resistance of plant life activities, so it has been extensively studied in recent years. This study retrieved from the NCBI protein database and download 29 wheat (Triticum aestivum) SOD protein sequence, and uses bioinformatics method, the proteins will be pided into 3 families according to the homologous degree. The physical and chemical properties, subcellular localization, conserved domains, signal peptide, spherical zone, disordered region, the secondary structure prediction, tertiary structure modeling and evolutionary analysis were proformed. The results showed that，there are obvious differences in A group SOD protein, B group SOD protein and C group SOD protein in physicochemical properties, secondary structure. Class A and B protein in different subfamily of sequences with low differentiation degree, the overall conservation is very high, the class C SOD proteins conserved low. SOD protein is high similarity to the Gramineae wheat SOD protein, the evolutionary is distance. The research results provide a theoretical basis for further study.
Key words：Triticum aestivm；Superoxide dismutase；Bioinformatics
目 录
摘 要 1
引 言 2
1 材料和方法 3
1.1 小麦超氧化物歧化酶蛋白序列的获得 3
1.2 小麦超氧化物歧化酶的生物信息学分析 3
1.2.1 基本理化性质分析 3
1.2.2 亚细胞定位、跨膜结构域、保守域、信号肽及紊乱区分析 3
1.2.3 二级结构预测 3
1.2.4 三级结构预测及进化分析 4
2 结果与分析 4
2.1 小麦超氧化物歧化酶蛋白同源性比较和聚类分析 4
2.2 生物信息学分析 6
2.2.1 小麦SOD蛋白理化性质分析 6
2.2.2 小麦SOD蛋白的跨膜结构域预测 6
2.2.3 小麦SOD蛋白的保守域预测 7
2.2.4 小麦SOD蛋白的信号肽预测 7
2.2.5 三种小麦SOD蛋白的紊乱区、球形区预测 9
2.3 二级结构分析 10
2.4 三级结构预测 11
2.5 对三级结构预测结果的评价 11
2.6 小麦SOD蛋白的进化树构建 12
2.6.1 小麦SOD蛋白的水平进化分析 12
2.6.2 小麦SOD蛋白的垂直进化分析 13
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