怀菊花活性成分及抑菌作用研究
关键词：怀菊花；活性成分；多糖；总黄酮；抑菌
Study on the Active Components and Antibacterial Activity
Of Huai Chrysanthemum
Abstract: In this experiment, the polysaccharide and total flavonoids of Huai chrysanthemum were extracted by hot-water and microwave-assisted extraction (MAE), and its contents were determined by spectrophotomety. At the same time, the antibacterial effects on bacterials such as Escherichia coli and Agrobacterium tumefacien were detected. The results showed that the extraction ratio of polysaccharide through hot-water extraction and MAE were 28.70 mg/g and 197.2 mg/g, respectively. The extraction ratio of total flavonoids with 75% methanol, 75% ethanol, 75% acetone and distilled water as extracting agent were 2.43 mg/g, 55.00 mg/g, 2.01 mg/g, 1.82 mg/g, respectively. It was also found that microwave-assisted extracts had better inhibition effects on Escherichia coli and Agrobacterium tumefacien with 0.69 cm of the inhibition zone diameter for the two bacterials.
Key words: Huai chrysanthemum; Active components; Polysaccharide; Total flavonoids; Antibacterial activity
目 录
摘 要 1
引言 2
1.实验部分 2
1.1 实验所用材料、仪器及试剂 2
1.2 怀菊花多糖的提取和含量测定 2
1.3 怀菊花黄酮的提取及含量测定 4
1.4 怀菊花提取液的抑菌活性 6
2.结果与分析 6
2.1 怀菊花多糖含量的测定 6
2.2 怀菊花黄酮含量的测定 7
2.3 怀菊花提取液的抑菌活性 8
3.结论 8
参考文献 9
致谢 11,3705
引言
菊花为多年生草本植物，以产于河南焦作一带的怀菊花最为有名，属于药用植物[1]，性甘、苦、凉。现代的医学研究证明，怀菊花有很好的抗菌效果，能散风清热、平肝明目、解毒消肿，既可内服，又可外用[2]。怀菊花内具有黄酮类、多糖、氨基酸、蛋白质、挥发油等成分[3]。多糖作为怀菊花的重要活性成分，具有降血糖、抗炎、抗肿瘤、清除自由基等作用。近年来，医药及科研单位研究发现怀菊花具有丰富的黄酮类物质，这类物质有扩张冠状动脉及增加冠状动脉的血流量的作用，对治疗心绞痛有一定效果。用怀菊花浸出物治疗冠心病患者，有效率达58%以上，尤其对伴有高血压、心绞痛的患者，有很好的疗效[4]。药理研究证实，菊花有广普抗菌作用，对多数皮肤真菌、葡萄球菌等具有明显的抑制效果。
本实验以怀菊花为实验材料，通过热水浸提和微波辅助提取两种方法来研究怀菊花多糖和黄酮的含量，并通过滤纸片法研究怀菊花多糖和黄酮提取液对常见致病菌大肠杆菌、农杆菌的抑制作用，以期为更有效的开发使用怀菊花提供参考。
1.实验部分
1.1实验所用材料、仪器及试剂
材料：怀菊花（购于周口七一路同和堂大药房）。
试剂：苯酚、NaNO2、Al(NO3)3、蒸馏水、甲醇、乙醇、丙酮、浓硫酸、氯仿、正丁醇。
上一篇：几株乳酸菌产酸性能的差异比较