摘要目的：本文旨在通过研究槲皮素对人类肺癌A549细胞系有没有抑制作用，来研究槲皮素对细胞生长抑制的机制，对研发新的抗癌药物和临床治疗肺癌具有指导意义。

方法：细胞复苏培养;倒置显微镜下观察细胞形态；通过划痕试验，观察不同浓度槲皮素处理后细胞的迁移情况；PI单染法检测细胞周期，对细胞周期分布进行分析；DPPH自由基清除活性实验检测槲皮素的抗氧化活性。73440

结果：槲皮素处理A549后，可以显著抑制细胞的生长；划痕实验的结果也显示，25 uM和50 uM的槲皮素处理细胞24和48小时后，均可以抑制细胞的迁移，而且这种作用具有浓度依赖性；DPPH自由基清除实验显示，槲皮素具有显著的清除自由基能力；此外，细胞周期检测表明，槲皮素可以明显提高A549细胞中S期的含量。

结论：槲皮素可以抑制肿瘤A549细胞的生长和迁移，这一作用与抗氧化活性和S期细胞阻滞有关。

毕业论文关键词：肺癌A549细胞、槲皮素、细胞迁移、自由基清除、细胞周期

Study of quercetin on the growth of human lung cancer A549 cells

Abstract Objective: This article aims to investigate whether quercetin can inhibit the growth of human lung cancer A549 cell, and to study the underlying mechanisms to provide theoretical support for the development of new anti-cancer drugs and the clinical therapy for lung cancer。 

Methods: Cell recovery culture and passage; Cell morphology was observed under inverted fluorescence microscope; The migration of cells under different concentrations was analyzed by scratch test; PI staining and flow cytometry were used to detect cell cycle; The anti-oxidation activity was measured by DPPH radical scavenging activity test。

Results: Quercetin inhibited the growth of A549 cells。 The results of scratch test also showed that after treated with 25 and 50 uM quercetin for 24 and 48 hours, the migration of A549 cells was suppressed dramatically, and this inhibitory effect was dose dependent; In addition, quercetin exhibited strong anti-oxidative activity as shown in DPPH radical scavenging test; Furthermore, by analyzing cell cycle, we found that quercetin elevated cell numbers in S phase。

Conclusion: Quercetin inhibits proliferation and migration of A549 cells by clearing up free radicals and blocking cell cycle in S phase。 

Keywords: Lung Cancer A549 cells; quercetin; cell migration; anti-oxidation; cell cycle

目录

摘   要 3

1前言 1

1。1肿瘤细胞 1

1。2槲皮素 1

1。3 PI单染法检测细胞周期 1

1。4细胞增殖 1

1。5 A549细胞 1

2 材料与方法 2

2。1材料 2

2。1．1器材 2

2。1。2材料 2

2。1。3试剂 3

2。2方法 4

2。2。1A549细胞的培养 4

2。2。2 用倒置显微镜观察细胞 5

2。2。3 A549细胞的划痕实验 5

2。2。4含槲皮素的培养液上清氧化活性的检测 5

2。2。5流式细胞仪检测细胞周期 6

2。2。6 统计学分析