摘要通过静电纺丝和己二胺胺解PLLA相结合的方法，得到氨基修饰的多孔POM/PLLA纤维。并通过SEM、TEM、XPS、FTIR等对其结构形貌进行了考察。将氨基化POM/PLLA多孔纤维用作脂肪酶固定化的载体，确定脂肪酶在纤维膜上固定化最优条件：酶溶液浓度为1。0mg/mL，固定时间为12h。通过酶催化橄榄油乳液水解反应，确定固定化酶的最适温度为40℃，最适pH=5。0。在最适条件下固定化酶催化橄榄油乳液水解反应的最大反应速率为232。56U/mg，米氏常数为11。70 mg/mL。相比于游离酶，固定化酶的催化活性、热稳定性及重复使用性大幅提高。89067

毕业论文键词：聚甲醛； 聚乳酸； 胺解反应； 脂肪酶固定化； 

Abstract The amino-modified porous POM / PLLA fibers were obtained by the combination of electrospinning and hexamethamine amination of PLLA。 The structure and morphology of the composites were investigated by SEM, TEM, XPS and FTIR。 The optimized conditions were as follows: the concentration of enzyme solution was 1。0 mg / mL and the fixation time was 12 h。 The optimal conditions for the immobilization of lipase on the fibrous membrane were as follows: the concentration of enzyme was 1。0 mg / mL and the fixation time was 12 h。 The optimal temperature of the immobilized enzyme was determined to be 40 ° C and the optimum pH was 5。0, by enzymatic hydrolysis of olive oil。 The maximum reaction rate of the olive oil emulsion was 232。56U / mg and the Michaelis - Menten constant was 11。70 mg / mL under the optimum conditions。 Compared to free enzyme, immobilized enzyme catalytic activity, thermal stability and reusability increased significantly。

Keyword: POM； Polylactic acid； Amination reaction； Lipase immobilization

目  录

摘  要 I

Abstract I

目  录 II

第1章 引言 1

2。1  原料与试剂 2

2。2  POM/PLLA-NH2的制备 2

2。3  脂肪酶在POM/PLLA-NH2表面固定化 2

2。4  结构与性能表征 2

(1) 结构表征 2

(2) 载酶量源Y于U优I尔O论P文W网wwW.yOueRw.com 原文+QQ75201-8766 和固定化效率 3

(3)固定化酶活性测定[8] 4

(4)固定化酶热稳定性测定 4

(5) 固定化酶的重复使用性 5

3。1  POM/PLLA纤维膜胺解改性 6

3。2 固定化酶的结构表征 9

3。3 脂肪酶固定化条件的研究 10

(1) 酶浓度对酶固定化率和比活性的影响 10

(2) 酶固定化时间对酶固定化率和比活性的影响 11

3。4固定化酶的催化性能研究 11

(1) 催化反应的最适pH 11

(2) 催化反应的最适温度 12

(3) 固定化酶促反应动力学 13

(4) 固定化酶促反应热力学 14

3。5 固定化酶的热稳定性 15

3。6 固定化酶的重复使用性 16

第4章 小结