摘要:纳米介孔二氧化硅（Mesoporous Silica Nanoparticles，MSN）凭借其较大的比表面积、规则的孔道、孔体积及孔径的可调节性和表面的易修饰等特点受到诸多研究小组的关注。在医学领域，作为药物载体，减少对人体的毒副作用以及药物可控释放的性能的研究更是成为研究热点。本课题采用溶胶-凝胶法通过有机模板诱导自组装合成MCM-41型介孔二氧化硅纳米颗粒。以介孔二氧化硅为内核，外层包裹交联聚甲基丙烯酸（PMAA）外壳，合成MSNs-PMAA复合材料。采用扫描电镜（SEM）、氮吸附（BET）、粒径分析等对MSNs和MSNs-PMAA样品表征进行性能对比。基于以上基础，对比MSNs被PMAA修饰前后装载阿霉素（DOX）的药物释放行为，同时做细胞毒性测试，看是否对人体细胞有毒副作用。还将两种材料所对应的载药微球与MCF-7进行共培养，探究两种载药系统对癌细胞的灭杀情况。62215

结果表明，相比于纯MSNs，经过PMAA修饰后的MSNs纳米微球具有更小粒径、更大孔容和更高的比表面积等特点，而且装载DOX后表现出更加优异的药物缓释性能，作用时间更长，能够较好的灭杀和抑制癌细胞。

毕业论文关键词：介孔二氧化硅；聚甲基丙烯酸；药物载体；溶胶-凝胶

Drug release behavior of pH sensitive mesoporous SiO2 based on PMAA modification

Abstract: Mesoporous Silica Nanoparticles (MSNs) has been concerned by many research teams owing to its large specific surface area, regular pore shape, pore volume and pore size, and easy to be modified. As a carrier to reduce the toxicity of the drug to the human body, the drug release behavior of a drug carrier has become a hotspot of research in the field of medicine. In this paper, the sol-gel method was used to induce the synthesis of MCM-41 mesoporous silica nanoparticles via organic template. With mesoporous silica as the core, the outer layer is covered with crosslinked Polymethacrylic acid (PMAA), and the composite material of MSNs-PMAA is fabricated. Scanning electron microscopy (SEM), nitrogen adsorption (BET) and surface analysis were utilized to compare the physic-chemistry properties of MSN and MSNs-PMAA nanoparticles. .Besides, the influence of PMAA modification to the drug loading content, the release behavior of the MSNs was also analyzed with the doxorubicin (DOX) as a model drug.On the other hand, to investigate the anti-cancer effect of these two drug delivery systems, the materials were co-cultured with MCF-7 cells and determined the quantity of cells by means of Microplate Reader.

The results show that compared to the pure MSNs, PMAA modified MSNs nanoparticles were shown the bigger size with and larger pore volume and higher specific surface area, as well as DOX loading, which showed more excellent drug release properties and better anticancer properties.

Key words: Mesoporous Silica Nanoparticles; Polymethacrylic Acid; Pharmaceutical Carrier; Sol-gel Method

目 录

摘要.i

Abstract..ii

1 绪论 1

1.1 引言 1

1.2 纳米靶向载药系统(Nano-targeting drug delivery system, NDDS ) 1

1.2.1 纳米药物载体的定义 1

1.2.2 纳米药物载体的分类 2

1.2.3 纳米药物载体的优势 2

1.3 介孔二氧化硅纳米颗粒(Mesoporous Silica Nanoparticles，MSNs) 3

1.3.1 介孔二氧化硅合成原理 3

1.3.2 介孔材料的结构特性及研究进展 4

1.4 介孔材料的官能化 5

1.5 pH敏感型药物控制释放体系