摘要：优化丹参中隐丹参酮 及丹参酮Ⅱ A 2种活性成分的超声提取工艺。方法:以丹参酮ⅡA 与隐丹参酮提取量为指标，采用响应面分析法考察液料比、提取时间、乙醇体积分数、超声功率对丹参提取效率、DPPH清除率的影响，采用MODDE软件设计、优化最优提取工艺。利用 HPLC 测定指标成分含量，流动相甲醇(A)～0. 2% 乙酸水(B)梯度洗脱(0 ～10 min，78% A；10～ 530min，78% ～ 85% A；30 ～ 35 min，85% A)，检测波长268nm，柱温：20℃,流速：0.8ml/min,进样量：10μl。结果：最佳超声提取工艺条件为乙醇体积分数 74%，超声功率 600W，液料比 1:5，提取时间 5min；隐丹参酮与丹参酮Ⅱ A 的提取量分别为0.140%，0. 192%，与2010 年版《中国药典》中加热回流法相比，活性成分提取量高于药典要求。结论:该超声提取工艺具有提取时间短、低毒、无污染等优点。70176

毕业论文关键词：响应面分析法；丹参；隐丹参酮；丹参酮ⅡA；超声提取

Application of Response Surface Analysis Method in Extraction Process of Traditional Chinese Medicine

Abstract: Optimization of Ultrasonic Extraction Technology of Two Kinds of Active Ingredients of Cryptotanshinone and Tanshinone Ⅱ A in Salvia Miltiorrhiza. Methods: The effects of the ratio of liquid to material, extraction time, ethanol volume fraction and ultrasonic power on the extraction efficiency and DPPH clearance rate of Salvia miltiorrhiza were studied by using the method of response surface analysis. The MODDE software was used to optimize the most Excellent extraction process. (0 ~ 10 min, 78% A; 10 ~ 530 min, 78% ~ 85% A; 30 ~ 35 (A) ~ 0.2% acetic acid (B) gradient elution (0 ~ 10 min, 78% A; Min, 85% A), detection wavelength 268 nm, column temperature: 20 ° C, flow rate: 0.8 ml / min, injection volume: 10 μl. Results: The optimum ultrasonic extraction conditions were as follows: ethanol volume fraction 74%, ultrasonic power 600W, liquid to material ratio 1: 5, extraction time 5min; the dosage of cryptotanshinone and tanshinone Ⅱ A was 0.132%, 0.188% 2010 edition of "Chinese Pharmacopoeia" in the heating reflux method compared to the active ingredient extraction is 

higher than the pharmacopoeia requirements. Conclusion: The ultrasonic extraction process has the advantages of short extraction time, low toxicity and no pollution.

Key words: response surface analysis method; Salvia miltiorrhiza; cryptotanshinone, tanshinone ⅡA, ultrasonic extraction process

目录

1 文献综述 2

1.1 丹参的基本介绍 2

1.1.1 资源分布 2

1.2 丹参的化学成分 2

1.2.1 丹参脂溶性成分 2

1.2.2 丹参水溶性成分 3

1.3 丹参的药理作用 5

1.3.1 对心血管的影响 5

1.3.2 抗肿瘤作用 5

1.3.3 其他作用 5

1.4 响应面优化法 6

2 实验内容 6

2.1 丹参中隐丹参酮和丹参酮ⅡA提取方法的选择 6

2.2 丹参脂溶性提取物抗氧化活性测定 7

2.2.1 实验部分 7

2.3 HPLC条件的优化 9

2.3.1 实验仪器与试剂 9

2.3.2 药材 10

2.3.3 色谱柱的选择