1.2.8TA克隆及测序
在PCR、琼脂糖凝胶电泳、胶回收后，接着连接0.25ulpMD18-T载体，转化                                                                                                                                                                                                                                                                                                                  大肠埃希菌 DH5α，然后筛选阳性克隆，送至上海生工检测基因顺序。 鲫鱼心源维隆气单胞菌的分离与鉴定(3):http://www.youerw.com/shengwu/lunwen_31835.html