摘要：Penicillium digitatum是果蔬采后主要的病原真菌，对果蔬的贮藏具有极大的危害，因此，寻找有效的防治方法具有重要的意义。本研究从腐烂的柑橘中分离主要致病菌，经rDNA-ITS法进行鉴定后，利用抑菌物质——癸醛处理病菌，确定最低抑菌浓度，并通过生理、生化指标以及果实接种实验，研究了癸醛与病原菌发育及致病力的关系。结果表明，分离的病原菌为P。 digitatum；癸醛的抑菌效果与浓度成正比；在最低抑菌浓度下，癸醛可以显著抑制P。 digitatum的孢子萌发，延缓芽管伸长及菌丝扩展速度，降低病原菌生物量积累，引起病原菌糖吸收障碍，并对柑橘绿霉病有明显的防治作用。本研究为果蔬采后病原菌P。 digitatum的防治提供了新的思路。74883

Abstract Abstract: Penicillium digitatum is a major postharvest pathogen and is quite harmful to fruit and vegetable preservation。 Therefore, it is necessary to search for an effective control method。 In this study, we isolated the main pathogen from naturally infected citrus and identified it by rDNA-ITS method。 Then minimum inhibitory concentrations (MICs) of exogenous antifungal substance (decanal) were confirmed。 The relationship between antifungal substance decanal and the growth or pathogenicity of the pathogen were evaluated via assessment of physiological, biochemical indexes and inoculation experiment of fruits。 The results indicated that the isolated pathogen was identified as Penicillium digitatum。 Decanal could inhibit the growth of Penicillium digitatum and the behavior was dose-dependent。 Furthermore, decanal with MICs could inhibit spore germination, reduce germ tube elongation and mycelium extension rate, lower biomass accumulation, result in sugar malabsorption of Penicillium digitatum, and effectively control green mold of citrus caused by Penicillium digitatum。 This study provides a new approach to control postharvest horticulture pathogens potentially of fruit and vegetable。

毕业论文关键词 ：Penicillium digitatum；癸醛；抑菌作用；贮藏保鲜

Keywords Penicillium digitatum; Decanal; Antifungal activity; Preservation and freshness

目   录

摘要 2

1引言 4

2 材料与方法 5

2。1菌株及主要试剂 5

2。2 rDNA-ITS法鉴定病原菌 5

2。3通过孢子萌发率确定最低抑菌浓度、P。 digitatum芽管长度的测量 5

2。4 P。 digitatum菌落扩展的测量 6

2。5菌丝生物量积累及总糖吸收率测定 6

2。6柑橘活体接种实验 6

2。7 数据统计与分析 7

3 结果与分析 7

3。1 P。 digitatum 的分离与鉴定 7

3。2通过P。 digitatum孢子萌发率确定癸醛最低抑菌浓度（MIC） 8

3。3癸醛（MIC）处理对P。 digitatum芽管伸长及菌落扩展的影响 9

3。4癸醛（MIC）处理限制P。 digitatum菌丝生物量积累并引起P。 digitatum糖代谢障碍 10

3。5癸醛（MIC）处理对P。 digitatum诱发的柑橘绿霉病具有明显的防治效果 10

4讨论 11

参考文献 12

致谢 14