摘要（（4-氯苯基）-（2-吡啶）-甲醇，CPMA）是倍它司汀的重要手性中间体，本论文是以（4-氯苯基）-（2-吡啶）-甲酮(CPMK)为底物，通过生物法催化转化得到产物(S)-CPMK。本课题建立在前人筛选得到能使（4-氯苯基）-（2-吡啶）-甲酮反应得(S)-CPMA的菌种的基础上，研究了游离细胞和固定化细胞在生物反应器中，生物催化水解生产S-CPMA的工艺过程。进过研究表明在本反应体系中，相对于培养罐体系，使用固定床生物反应器可以明显的提高反应的产率与ee值，在菌量为8 g，葡萄糖浓度为1。0 g/L，反应底物为1g/L，反应时间为24 h时可达到48。88% ,49。19%

论文研究了游离细胞在自行设计的固定床生物反应器中的转化工艺，优化了转化条件，在反应器中加入带有游离细胞的底物缓冲溶液，可以进行多次转化反应。并对时间进行反应条件的优化，发现在24 h时有理想的光学活性与产率组合，并在此时间上对葡萄糖浓度进行优化，在1。0 g/L时有理想的光学活性与产率组合，基于此条件又对反应底物进行优化发现在1。0 g/L时有理想的光学活性与产率组合，最后对反应菌量进行优化在8 g时有理想的光学活性与产率组合，最后确定反应的最佳时间24 h，最佳葡萄糖浓度1。0 g/L，最佳的反应底物量1。0 g/L与菌量8g。93952

毕业论文关键词:固定床生物反应器，固定化细胞，倍他司汀，（4-氯苯基）-（2-吡啶）-甲醇， （4-氯苯基）-（2-吡啶）-甲酮

ABSTRACT Betahistine (4 - chlorobenzene (base) - (2 - pyridine) - methanol, CPMA) is a kind of important drug intermediates, this thesis is based on 4 - chlorobenzene (base) - (2 - pyridine) - ketone (CPMK) as substrate, is obtained by biological catalytic conversion product (S) - CPMK。 This topic is based on the predecessors' screening can get make (4 - chlorobenzene) - (2 - pyridine) - ketone reaction to (S) - CPMA strains, on the basis of free and immobilized cells was studied in the bioreactor, biological catalytic hydrolysis production S - CPMA process。 Went to study in this reaction system, relative to the training tank system, using a fixed bed bioreactor can significantly improve the reaction yield and ee value, the amount of bacteria is 8 g, glucose concentration is 1。0 g/L, the reaction of the substrate is 1 g/L, the reaction time of 24 h can reach 48。88%, 49。19%

Paper studied the free cell in the design of a transformation process in fixed bed bioreactor, and optimized conditions, the reactor in the substrate buffer solution with free cells, can be repeatedly transfer reaction。 And the time for the optimization of reaction conditions, found in 24 h are ideal optical activity and yield of combination, and optimize the glucose concentrations, in the time at 1。0 g/L ideal optical activity and yield combination, based on the conditions and optimize the reaction substrates found in 1。0 g/L have ideal optical activity and yield combination, finally optimize the microbial reactions in 8 g has the ideal combination of optical activity and yield, and finally determine the optimal time of 24 h of reaction, the optimal glucose concentration is 1。0 g/L, the best reaction substrate 1。0 g/L and bacteria amount 8 g。

KEY WORDS: Fixed bed bioreactor, immobilized cell, betahistine, 4 - chlorobenzene (base) - (2 - pyridine) - methanol, 4 - chlorobenzene (base) - (2 - pyridine) - ketone 

目录

摘要 2

ABSTRACT 2

1 引言 5

1。1固定化反应器 5

2 课题主要内容 5

2。1 实验仪器与药品 6

2。1。1 培养基配方