摘要毛细管电色谱（CEC）是近十几年来快速发展起来的一种新型的分离技术，它不仅兼具高效液相色谱的高选择性，而且还具有毛细管电泳的高效性。碳纳米材料（如石墨烯，氧化石墨烯，单壁碳纳米角等）作为吸附剂，已被广泛应用于样品的预富集。其中，石墨烯具有较大的比表面积、较强的π-π共轭效应和疏水作用，而氧化石墨烯表面含有大量的羟基、羧基以及环氧基等活性基团，具有更好的分散性、水溶性和可修饰等性能。单壁碳纳米角结构的独特性，在很多领域有潜在的应用价值。所以，石墨烯类材料及单壁碳纳米角有望成为毛细管电色谱中极具发展前景的固定相材料。75725

单壁碳纳米角（SWNHs）通过一步聚合反应修饰电色谱固定相，实验探究了流动相中缓冲溶液pH值对整体柱中电渗流的影响，流动相乙腈比例及分离电压对中性物质分离的影响。SWNHs修饰的毛细管整体柱对中性化合物和碱性化合物有较好的分离效果，并且重现性较好。

ABSTRACT    Capillary electrochromatography （CEC）is a new separation technology in recent years, which combines the high selectivity of high performance liquid chromatography (HPLC) and the high efficiency of  capillary electrophoresis (CE)。 Carbon nanomaterials (like graphen, graphene oxide, single-walled carbon nanotubes) , which could be used as sorbent materials had been widely used for preconcentration。 Graphen (G) has the ultrahigh specific surface area and strong π-π interactions。 Compared with G, graphene oxide (GO) is a kind of chemically modified graphene sheets containing a range of reactive oxygen functional groups, such as epoxy, hydroxyl and carboxyl, on the basal planes and edges。 Single-wall carbon nanohorns (SWNHs) have received great interest for its unique properties and potential perse applications。 So, the materials are expected to become the capillary electrochromatography of stationary phase material。

    We show the preparation of SWNHs embedded monolithic column via one-step polymerization for capillary electrochromatography (CEC)。 The influence of mobile phase composition (volume fraction of organic solvent in mobile phase, electrolyte) on the separation for neutral compound was subsequently examined。 Baseline separation of the tested neutral analytes (alkyl benzenes and polycyclic aromatics) on the SWNHs embedded monolithic column。 This work also demonstrated that highly efficient CEC separation in the SWNHs embedded monolithic columns is feasible。

毕业论文关键词：碳纳米角； 毛细管； 分离；电色谱； 整体柱

Keyword: carbon nanohorn; capillary separatio; capillary electrochromatography; monolithic column

目    录

单壁碳纳米角修饰毛细管电色谱整体柱的制备与性能研究 1

ABSTRACT 2

1。 引言 5

1。1。 纳米材料 5

1。2。 整体柱 5

1。3。 单壁碳纳米角 6

2。 实验部分 7

2。1。 仪器与试剂 7

2。2。 样品和流动相制备 8

2。3。 毛细管预处理 8

2。4。