摘 要：采用不同强度的紫外辐射处理蚕豆种子的主根及其侧根，研究其不同强度的紫外辐射对蚕豆种子的主根和侧根根尖细胞外表皮颜色、主根和侧根根尖有丝分裂指数、主根和侧根根尖细胞染色体畸变率、主根和侧根根尖细胞细胞微核率、侧根细胞根系活力等的影响。结果显示，在蚕豆培养其他条件一致的情况下，随着紫外辐射处理时间的延长，无论是主根还是侧根，根部外表皮颜色都明显发黑甚至有烂根现象，根尖的染色体畸变率、细胞微核率都随着辐射时间的延长而变大，而主侧根的有丝分裂指数和侧根根系活力却随着紫外辐射时间的延长而减弱；所以在蚕豆种子培养条件一致的前提下，紫外辐射强度与蚕豆主侧根根尖细胞的染色体畸变率、细胞微核率成正比，与蚕豆主侧根的有丝分裂指数和侧根根系活力成反比。上述分析说明，与对照组相比，不同强度的紫外辐射对蚕豆主侧根根尖细胞根尖的有丝分裂指数、染色体畸变率、细胞微核率、根尖外表皮颜色以及侧根根系活力均有不同程度的影响。85024

毕业论文关键词：蚕豆；紫外辐射；染色体畸变率；微核率；生理指标

Study on the genetic toxicity of ultraviolet radiation  on the root tip cells of broad bean

Abstract: Taproot and lateral roots with different strength of ultraviolet radiation treatment of bean seeds, study the different intensity of UV radiation of taproot and lateral root tip cells of Vicia faba seed skin color, taproot and lateral root apical mitotic index, taproot and root tip cell chromosome aberration rate of root, root and root tip cell micronucleus rate。 Effect of root cells of root activity。 The results showed that in the broad bean culture under same conditions, with increasing ultraviolet radiation treatment time, whether or root lateral root, root skin color are obvious and even black rot, root tip mitotic index, the rate of chromosome aberration, micronucleus rate along with the prolonging of irradiation time and change, while the side root system activity weakened; so the precondition of training consistent in broad bean seeds, ultraviolet radiation intensity and the main root of Vicia faba root tip The mitotic index, chromosome aberration, micronucleus cell rate is directly proportional to, and the lateral root and root activity is inversely proportional to the cell。 The analysis shows that compared with the control group, the different intensity of UV radiation on Vicia faba lateral root tip cells of root tip mitotic index and chromosome aberration rate and micronucleus rate and root skin color and root vitality were varying degrees of impact。

Key words: Broad bean; ultraviolet radiation; chromosome aberration rate; micronucleus rate; physiological index

目    录

摘 要 1

引言 2

1。 材料与方法 3

1。1 实验材料 3

1。2 实验方法 3

1。2。1 实验试剂制备 3

1。2。2 蚕豆种子萌发与生根 3

1。2。3 实验处理设计 3

1。2。4 指标测定 4

2。 结果与分析 5

2。1 不同强度的紫外辐射对蚕豆根尖细胞外表皮颜色的影响 5

2。2不同强度的紫外辐射对蚕豆根尖细胞有丝分裂指数的影响 5

2。3不同强度的紫外辐射对蚕豆根尖细胞染色体畸变率的影响