摘要：ECO板微培养板是将32个生化测试反应集中于一个微型测试板上集成平台，具有标准、体积小、使用方便的优点。

松材线虫病是林业上最危险的林业病害之一，松材线虫与其伴生细菌协同致病是近年来的研究热点。本论文首次将用不同方法分离的松材线虫伴生细菌尝试以ECO板微培养板进行数值化鉴别归类。研究结果如下：

（1）、用微孔变色指标可以方便的将不同系列的菌株加以归类，但可靠性仍需验证，置信区间仍需确认，本次将判定终值低于4（含4）的菌株汇总，其中WCCJ4h-N-a和WCCJ4h-P-c、WCCJ4h-N-a和WCCJ4h-P-f、WQX-3-N-d和WQX-3-P-e、NBQX-3-N-a和NBQX-3-P-a差异和为1，NCCJ4h-N-a和NCCJ4h-P-a差异和为0，因此，它们极有可能为同一种菌。但在上述存在包含或高度重叠的系列菌株中，少有比对完全一致的，仍是需要研究和探讨的。

（2）、用浊度数据对菌株进行比对，菌与菌之间逐一对比，均没有鉴定出两个菌株为同一种菌。因此，无法实现利用ECO板的浊度值来鉴定菌种和比对菌株。

关键词：细菌；鉴别；ECO板；

Abstract：ECO plate micro-culture plate will focused on a micro-test board integrated platform with 32 biochemical test response .The advantages of ECO plate is standard, small size and easy.Pine wood nematode disease is one of the most dangerous forestry diseases in forestry. The synergistic pathogenicity of Bursaphelenchus xylophilus and its associated bacteria is a hotspot in recent years. The paper will taken the ECO micro-culture plate to classify the bacteria associated with  Bursaphelenchus xylophilus used by different methods in the first time.The results as follows:

(1) With the micro-color change indicators can be easily different strains of strains to be classified.But the reliability still need to verify, confidence interval still need to confirm.The reseach will determine the final value of less than 4 (including 4) of the strains summarized.The difference between WCCJ4h-Na and WCCJ4h-Pc, WCCJ4h-Na   and   WCCJ4h-Pf,   WQX-3-Nd   and   WQX-3-Pe,   NBQX-3-Na  and NBQX-3-Pa is 1, NCCJ4h-Na and NCCJ4h -Pa difference is zero.Therefore, they are most likely to be the same species.

(2) The turbidity data were used to compare the strains, and the bacteria and bacteria were compared one by one. No strains were identified as the same bacteria.Therefore, it is not possible to use the turbidity value of the ECO plate to identify the strain and the comparative strain.

Key words:bacteria, identification, ECO plate

目录

第一章绪论 1

1.1松材线虫病 1

1.2抗病育种学研究 2

1.3松材线虫病原学的研究 3

1.4松材线虫病致病机理的研究 4

1.5松材线虫病的化学和物理防治研究 6

1.6病害传媒昆虫生物学及防治研究 8

第二章材料与方法 9

2.1实验菌种名称 9

2.2菌种来源 9

2.3实验材料 9

2.4实验方法与步骤 10

2.4.1培养基配制 10

2.4.2菌株的活化 10

2.4.4BIOLOGECO板点样 10

2.4.5培养观察和数据采集