外源亚精胺对高温胁迫下番茄幼苗抗氧化系统的影响
毕业论文关键词：番茄；Spd；高温胁迫；抗氧化系统
Effects of exogenous spermidine on antioxidant system of tomato seedlings exposed to high temperature stress
Abstract：This experiment which discussed the effects of foliar spraying spermidine on oxidative stress and antioxidant protective system of Tomato Seedlings under high temperature stress was carried out with "Pu Hong 968" tomato varieties in substrate culture.The results show that under high temperature stress, the ROS (O2.– production rate and H2O2 content) of tomato seedlings increased significantly, and the damage of membrane peroxidation was aggravated.Exogenous Spd treatment increased the activity of SOD, POD, CAT, APX, DHAR under high temperature significantly.Meanwhile, exogenous Spd significantly increased the content of antioxidants (AsA and GSH) in the leaves of seedlings,the proportion of AsA and GSH was also increased,so the intracellular redox balance was maintained.Results show that the exogenous Spd can activate antioxidant enzymes and non enzymatic antioxidant so that O2.– production rate,H2O2 content and MDA content will be decreased significantly.Oxidative stress induced by high temperature stress on Tomato Seedlings and lipid peroxidation will be reduced.
Key words: tomato; spermidine; high temperature stress; antioxidant system
目 录
摘要 1
关键词 1
Abstract 1
Key words 1
引言 1
1 材料与方法 2
1．1 材料与设计 2
1．2 测定及分析方法 3
1．2．1 活性氧产生 3
1．2．2 丙二醛含量和电解质渗透率 3
1．2．3 抗氧化酶活性 3
1．2．4 非酶抗氧化剂 3
2 结果与分析 3
2．1 外源亚精胺对高温胁迫下番茄幼苗活性氧产生的影响 3
2．2 外源亚精胺对高温胁迫下番茄幼苗膜脂伤害的影响 4
2．3 外源亚精胺对高温胁迫下番茄幼苗抗氧化酶活性的影响 4
2．4 外源亚精胺对高温胁迫下番茄幼苗非酶抗氧化剂含量的影响 5
3 讨论 7
致谢 8
参考文献 8
图1 外源Spd对高温胁迫下番茄幼苗叶片H2O2含量和O2.–产生速率的影响 3
图2 外源Spd对高温胁迫下番茄幼苗叶片MDA含量和电解质渗透率的影响 4
图3 外源Spd对高温胁迫下番茄幼苗叶片SOD、CAT和POD活性的影响 4
图4 外源Spd对高温胁迫下番茄幼苗叶片APX、GR、MDHAR和DHAR活性的影响 5