不同氮素水平对盐胁迫下菘蓝幼苗生长和活性成分的影响
毕业论文关键词：菘蓝；氮素水平；盐胁迫；幼苗生长；活性成分
Effects of different nitrogen levels on seedling growth and active components of Isatis indigotica Fort. under salt stress
Abstract： Objection: To explore the effects of different nitrogen levels on seedling growth and active components of Isatis indigotica Fort. under salt stress. Method: The Hebei baoding cultivation population at the seedling stage were used as materials. The changes of seedling growth, protective enzyme and nitrogen metabolism enzyme activities, protine contents and accumulation of active ingredients were investigated by supplying with different nitrogen levels (T1:3.75 mmol•L-1, T2:7.5 mmol•L-1, T3:15 mmol•L-1, T4:18.75 mmol•L-1, T5:22.5 mmol•L-1) under 100 mmol•L-1NaCl stress, and the normal nitrogen level(15 mmol•L-1) without salt was taken as the comparison(CK). Result: It was demonstrated that the fresh and dry weights of leaf and root of I. indigotica seedling initially increased and then decreased. Both the lower (T1 and T2) and much higher (T5) nitrogen application under salt stress could decrease the activities of antioxidant enzymes-SOD, POD, CAT and protein contents, while increase the MDA contents in I. indigotica leaves. Conclusion: The appropriate nitrogen application could promote the biomasses, antioxidant enzyme activities and osmoregulation abilities of I. indigotica under salt stress. Besides, it could also improve the accumulation of soluble sugar, soluble protein, free amino acid and epigoitrin. In conclusion, the appropriate nitrogen application (T4) could release the harm of salt stress to the I. indigotica seedling growth and promote the accumulation of active components.
Key words: Isatis indigotica Fort.; nitrogen level; salt stress; seedling growth; active component
目 录
摘要 1
关键词 1
Abstract 1
Key words 2
引言 2
1 材料与方法 3
1．1 试验材料与方法 3
1．2 测定项目与方法 3
1．2．1 生物量的测定 3
1．2．2 抗氧化酶活性的测定 3
1．2．3 丙二醛（MDA）含量的测定 3
1．2．4 脯氨酸含量的测定 3
1．2．5 活性成分的测定 3
1．2．6 氮代谢关键酶活性和硝态氮含量的测定 3
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