摘要:本试验旨在研究日粮添加姜黄素对宫内发育迟缓(intrauterine growth retardation, IUGR)鼠肌肉抗氧化机制的影响。试验采用2×2因子设计,12只正常仔鼠和12只IUGR仔鼠21日龄断奶后随机分成四个处理组(每组6只):NBW组为正常仔鼠,饲喂基础日粮;NC组为正常仔鼠,饲喂基础日粮+400 mg/kg姜黄素;IUGR组为IUGR仔鼠,饲喂基础日粮;IC组为IUGR仔鼠,饲喂基础日粮+400 mg/kg姜黄素。断奶适应期1周,正式试验期4周。结果显示:(1)与NBW组相比,IUGR组大鼠肌肉丙二醛(malondialdehyde, MDA)含量显著升高(P < 0.05),谷胱甘肽过氧化物酶(glutathione peroxidase, GSH-PX)活力显著降低(P < 0.05)。日粮添加姜黄素可显著降低IUGR大鼠肌肉MDA含量(P < 0.05),显著升高总超氧化物歧化酶(totalsuperoxide dismutase, T-SOD)活力(P < 0.05);(2)与NBW组相比,IUGR组大鼠肌肉核因子NF-E2相关因子2(nuclear factor erythroid-2 related factor 2, Nrf2)、超氧化物歧化酶1(superoxide dismutase, SOD1)基因mRNA的表达水平均显著降低(P < 0.05)。日粮添加姜黄素可显著提高IUGR组大鼠肌肉Nrf2、SOD1基因mRNA的表达水平(P < 0.05)。试验结果表明,日粮添加400 mg/kg姜黄素可有效缓解IUGR引起的大鼠肌肉氧化损伤。30508
毕业论文关键词:姜黄素;IUGR;抗氧化
Effects of curcumin on anti-oxidation in the muscle of IUGR rats
Abstract: The present study was designed to evaluate the effects of dietary supplementation of curcumin on anti-oxidation in the muscle of IUGR (intrauterine growth retardation) rats. Twelve normal and twelve IUGR newborn rats were randomly assigned to the following four experimental groups (n = 6/group): NBW group (normal rats, fed with basic diets), NC group (normal rats, fed with basic diets + 400 mg/kg curcumin), IC group (IUGR rats, fed with basic diets) and IC group (IUGR rats, fed with basic diets + 400 mg/kg curcumin). Rats were fed from 4 to 8 weeks of age,including a one-week adaptive phase for weaning. The results indicated that: (1) compared with NBW group, IUGR rats had a higher content of MDA (malondialdehyde) in the muscle (P < 0.05). Furthermore, the activities of GSH-PX(glutathione peroxidase) was significantly decreased in muscle of the IUGR rats (P < 0.05) as compared with that of NBW group. Curcumin significantly reduced the content of MDA (P < 0.05), whereas increased the activities of T-SOD (totalsuperoxide dismutase) in the muscle of IUGR rats (P < 0.05); (2)compared with NBW group, the mRNA expression of Nrf2 (nuclear factor erythroid-2 related factor 2) and SOD1 in the muscle of IUGR rats were significantly down-regulated (P < 0.05). However, curcumin up-regulated these mRNA expressions (P < 0.05). In conclusion, 400 mg/kg curcumin can effectively alleviate the damage by IUGR and had beneficial effects on the oxidative damage in the muscle of IUGR rats.  
Key words: Curcumin; IUGR; anti-oxidation
目  录
摘要    1
Abstract    1
key words    2
引言    2
1 材料与方法    2
1.1  试验设计    3
1.2  饲养管理    3
1.3  样品采集    3
1.4  测定指标及方法    3
1.4.1  肌肉组织抗氧化及氧化相关指标测定    3
1.4.2  肌肉组织中目的基因mRNA表达量测定    3
1.5  数据统计与分析    4
2  结果与分析    4
2.1  日粮添加姜黄素对正常和IUGR大鼠肌肉抗氧化系统的影响    4
2.2  日粮添加姜黄素对正常和IUGR大鼠肌肉抗氧化相关基因表达的影响    5
3  讨论    6
上一篇:基于Akt信号通路研究黄体期营养水平及静脉注射葡萄糖对湖羊卵泡发育的影响
下一篇:复合益生菌发酵饲料菌种配伍筛选

植物生长素响应因子ARF1...

稻田锌含量对水稻害虫褐...

甲苯二甲苯对斑马鱼幼鱼神经的毒性影响

PEG6000模拟干旱胁迫对大麦...

教育实习前后生物专业师...

小鼠皮层PV+神经元fgfr2敲除对突触蛋白的影响

重金属铬对白尾灰蜻稚虫...

公寓空调设计任务书

10万元能开儿童乐园吗,我...

志愿者活动的调查问卷表

神经外科重症监护病房患...

C#学校科研管理系统的设计

承德市事业单位档案管理...

国内外图像分割技术研究现状

中国学术生态细节考察《...

医院财务风险因素分析及管理措施【2367字】

AT89C52单片机的超声波测距...