姜黄素对IUGR鼠肌肉抗氧化机制的研究
毕业论文关键词：姜黄素；IUGR；抗氧化
Effects of curcumin on anti-oxidation in the muscle of IUGR rats
Abstract: The present study was designed to evaluate the effects of dietary supplementation of curcumin on anti-oxidation in the muscle of IUGR (intrauterine growth retardation) rats. Twelve normal and twelve IUGR newborn rats were randomly assigned to the following four experimental groups (n = 6/group): NBW group (normal rats, fed with basic diets), NC group (normal rats, fed with basic diets + 400 mg/kg curcumin), IC group (IUGR rats, fed with basic diets) and IC group (IUGR rats, fed with basic diets + 400 mg/kg curcumin). Rats were fed from 4 to 8 weeks of age，including a one-week adaptive phase for weaning. The results indicated that: (1) compared with NBW group, IUGR rats had a higher content of MDA (malondialdehyde) in the muscle (P < 0.05). Furthermore, the activities of GSH-PX(glutathione peroxidase) was significantly decreased in muscle of the IUGR rats (P < 0.05) as compared with that of NBW group. Curcumin significantly reduced the content of MDA (P < 0.05), whereas increased the activities of T-SOD (totalsuperoxide dismutase) in the muscle of IUGR rats (P < 0.05); (2)compared with NBW group, the mRNA expression of Nrf2 (nuclear factor erythroid-2 related factor 2) and SOD1 in the muscle of IUGR rats were significantly down-regulated (P < 0.05). However, curcumin up-regulated these mRNA expressions (P < 0.05). In conclusion, 400 mg/kg curcumin can effectively alleviate the damage by IUGR and had beneficial effects on the oxidative damage in the muscle of IUGR rats.
Key words: Curcumin; IUGR; anti-oxidation
目 录
摘要 1
Abstract 1
key words 2
引言 2
1 材料与方法 2
1.1 试验设计 3
1.2 饲养管理 3
1.3 样品采集 3
1.4 测定指标及方法 3
1.4.1 肌肉组织抗氧化及氧化相关指标测定 3
1.4.2 肌肉组织中目的基因mRNA表达量测定 3
1.5 数据统计与分析 4
2 结果与分析 4
2.1 日粮添加姜黄素对正常和IUGR大鼠肌肉抗氧化系统的影响 4
2.2 日粮添加姜黄素对正常和IUGR大鼠肌肉抗氧化相关基因表达的影响 5
3 讨论 6
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