以酒糟为原料的发酵TMR配制及发酵调控
毕业论文关键词：酒糟；添加剂；发酵TMR；发酵品质；有氧稳定性
The formulation and modulation of fermentation of total mixed ration prepared with distillers’ grains
Abstract : Distillers’ grains is a kind of nutritious by-products with a great quantity.However, the seasonal supply , high moisture content which makes distillers’ grains difficult to store and other characteristics limit the comprehensive utilization of distillers’ grains.The objective of this study was to process distillers’ grains to forage by the form of fermentation total mixed ration (FTMR) .First we processed distillers’ grains with the concentrate to FTMR in different proportions, The results showed that different proportions distillers’ grains after silage 90 days had a significant influence on pH value（P<0.001）,lactic acid（P<0.001）,acetic acid（P<0.001）,propionic acid（P<0.001）, butyric acid（P<0.001）, ethanol ammonia nitrogen/total nitrogen（P<0.001）, water soluble carbohydrates（P<0.001）.Compared to the control group,25% distillers’ grains and 35% distillers’ grains had lower pH ,content of acetic acid and ethanol ammonia nitrogen/total nitrogen,while the content of water soluble carbohydrates was higher.From the perspective of maximizing the use of distillers’ grains.35% distiller's grains was selected to do the next research,controlling the fermentation quality and aerobic stability of TMR by adding potassium sorbate, sodium benzoate, calcium propionate and sodium diacetate.Each of the TMR was exposed to air for 90 days and evaluated for its aerobic stability.As the silage carried out,the pH value of each group increased gradually, the group adding potassium sorbate and sodium benzoate slowly,8 days before aerobic exposure,up to 4.80 and 5.60 respectively,while the other groups rose to more than 6.0,then,Until the end of silage,the sorbic acid group rose slightly to 5.15, while the sodium benzoate group significantly increased to 6.09.The content of ethanol ammonia nitrogen/total nitrogen of every treatment with additives was lower than control group,and the calcium propionate group and sodium diacetate group were lower.With the prolongation of aerobic exposure time, the lactic acid content of each group decreased gradually, but the decrease rate of lactic acid content in each treatment group was significantly lower than control group.The content of acetic acid and propionic acid in each group decreased gradually,only minor amounts of butyric acid were detected in each group during exposure.In this experiment, the ethanol content of each group gradually increased,but the ethanol content of each additive treatment group was always lower than that of control group,which indicate the antifungal properties of each additive.To sum up,the FTMR with 35% distillers’ grains has good fermentation quality,and the Aerobic stability was increased by adding 0.1% potassium sorbate, pH value could still remained at 5.15 after exposure for 9 days.