拟南芥甘油-3-磷酸酰基转移酶(AtGPAT)基因的生物信息学分析
毕业论文关键词：油脂合成；AtGPAT；生物信息学；电子定位；表达量
Bioinformatics Analysis of GPAT Proteins in Arabidopsis thaliana
Abstract: Sn-glycerol-3-phosphate acyltransferase(GPAT) catalyzes the acylation at sn-1 position of glycerol-3- phosphate to produce lysophosphatidic acid (LPA) in an acyl-CoA or acyl-ACP-dependent manner，which is the initial and rate determining step of TAG biosynthetic pathway.We download protein sequences of Arabidopsis thaliana GPAT from the NCBI database. This paper, by using bioinformatics methods and techniques, basic physical and chemical properties, subcellular localization of AtGPATs were analyzed. Further more, secondary, tertiary structure and evolutionary analysis was carried out to understand the related information between structure and function. The results showed that the AtGPAT proteins were clustered into 4 groups, and significant difference was found among the four selected AtGPAT protein amino sequences in length.and no significant difference in amino acid composition. The secondary structure was mainly α-helix and random coil, with less beta angle. The tertiary structure of BAA00576.1 was predicted, which meets the rule of stereochemistry in the figure of Ramachandran. It was also showed thatthis gene specifically expressed in the seed of Arabidopsis thaliana.
Keywords: Lipid biosynthesis; AtGPAT;Bioinformatics; Electronic mapping; Expression level
目 录
摘要 1
引言 2
1.材料和方法 3
1.1材料来源 3
1.2 拟南芥GPAT蛋白生物信息学分析 3
2.结果与分析 3
2.1 拟南芥 GPAT蛋白序列的获取和进化分析 3
2.1.1 拟南芥GPAT蛋白序列获取 3
2.1.2 拟南芥GPAT蛋白序列进化分析 3
2.1.3 植物GPAT蛋白序列进化分析 7
2.2 生物信息学分析 9
2.2.1 基本理化性质分析 9
2.2.2 蛋白质跨膜区、亚细胞定位、磷酸化分析 11
2.3 拟南芥GPAT蛋白二级结构预测分析 13
2.3.1 拟南芥GPAT蛋白二级结构及保守域分析 13
2.3.2 蛋白紊乱区、球蛋白区预测 14
2.4 蛋白质三级结构的预测分析 16
2.4.1 蛋白质三级结构的预测 16
2.4.2 对三级结构预测结果的评价 16
2.5 拟南芥GPAT蛋白基因结构与定位分析 18
2.6 拟南芥GPAT蛋白电子表达分析 18
3.结论与讨论 20
参考文献 20
致 谢 22
拟南芥甘油-3-磷酸酰基转移酶(AtGPAT)基因的生物信息学分析 引言
油脂是人类非常重要的一种食物，也是很多化工产品的原料。现在全球经济发展的越来越快，人类对能源的需求量也日益加大。伴随着石油资源的逐渐耗尽，人们开始重视植物油脂被作为生物燃料的可能性。植物油脂主要贮存于种子或者果实中，以TAG的形式存在，在叶片和其他营养组织中也有少量存在。从经济的角度看，种子含油脂量越高，那么经济作物产量就越高。
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