橙花醇对白色念珠菌的细胞周期和细胞凋亡的影响
毕业论文关键字:橙花醇；白色念珠菌；细胞周期；细胞凋亡
Effects of Nerol on the Cell Cycle and Cell Apoptosis of Candida albicans
Abstract：Nerol, a colorless oily liquid, smell beautiful and soft, and relatively clear, is a valuable spice and pharmaceutical intermediates, in essence, medicine, food, cosmetic and other industries has extensive application value. C. albicans, also known as white Candida yeast, is a common condition pathogenic fungi. The microdilution was applied to detect MIC of C. albicans which treated by nerol according to M38-A2 method of NCCLS, the MIC is 0.05 µL/mL. The change of ergosterol content was determined by ultraviolet spectrophotometer. The cell cycle and apoptosis of C. albicans treated by nerol was detected by flow cytometry. the results show that the nerol can inhibit the growth of C. albicans by effect it’s logarithmic growth phase cells, and on the cell apoptosis, nerol lead to apoptosis by make the phosphatidylserine outside the membrane.
Key words: Nerol； C. albicans； Cell Cycle； Cell Apoptosis
目录
引言 1
1 材料与方法 3
1.1供试材料 3
1.1.1 原料 3
1.1.2 主要试剂 3
1.1.3主要仪器 4
1.1.4培养基 5
1.1.5试剂溶液的配制 5
1.2 实验方法 6
1.2.1 白色念珠菌的菌株活化 6
1.2.2 菌悬液的制备 6
1.2.3 微孔稀释法测定最低抑菌浓度值(Minimum Inhibitory Concentration,MIC) 6
1.2.4麦角固醇含量分析(Sterol Quantitation Method,SQM) 6
1.2.5 橙花醇对白色念珠菌细胞膜损伤的影响 7
1.2.6 橙花醇对白色念珠菌细胞周期的影响 7
1.2.7 橙花醇对白色念珠菌细胞凋亡的影响 8
2 结果与分析 8
2.1 橙花醇的最低抑菌浓度分析(Minimum Inhibitory Concentration, MIC) 8
2.2麦角固醇含量分析(Sterol Quantitation Method, SQM) 9
2.3橙花醇对白色念珠菌细胞膜损伤的影响 9
2.4橙花醇对白色念珠菌细胞周期的影响 13
2.5橙花醇对白色念珠菌细胞凋亡的影响 15
3 讨论 17
4 致谢 19
参考文献 19
引言
现今，由于免疫抑制剂、广谱抗生素和细胞毒药物的大量应用；器官移植、静脉营养和导管介入等治疗手段的普及；糖尿病、恶性肿瘤、血液病、艾滋病、老年病等临床严重疾病的发生率的不断上升[1]，使得真菌感染，尤其是条件真菌感染的发病率及死亡率逐年上升[2]。
白色念珠菌(C. albicans)，又名白色假丝酵母菌，酵母科念珠菌属，是常见的条件致病性真菌[3]，是人体皮肤、粘膜的正常寄生菌，可引起人黏膜、皮肤、消化道、呼吸及神经系统等感染[4]，人体免疫力低下时会引起浅表性的或深部的感染，严重者会引起系统性感染，将危及生命[5]。
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