油菜胁迫蛋白SAP家族成员挖掘与鉴定
毕业论文关键词：油菜；SAP蛋白；基因结构；进化分析
Discovery and Identification of Stress-associated Protein of SPA Family Members in Brassica Rape L
Abstract: Abiotic stresses such as chilling injury, damage of drought, salinity and plant diseases and insect pests, is harmful to plant growth and development. Greater degree of harm and serious abiotic stress will be caused serious economic losses on rape and other crops, which affecting crop production. With abiotic stresse, crops would be induced to produce varieties of protein to adapt to the stress pressure, the SAP (stress-associated protein) proteins induced by abiotc stress have important roles on responses of stress and the regulation of stress. Therefore, it is one of the hot topics in botanical research to tap and identify new stress-related proteins. In order to obtain the information of stress-associated protein of rape. This study were retrieved 27 different stress-associated protein sequences of cabbage from NCBI protein sequence database, then by biological information technology and the methods, we got basic physical and chemical information, structure information, location, protein sequences conserved domains and evolutionary information. The results showed that, the SAP protein family in rape has 27 diferent members (excluding consistent sequences), distributed in from I to IX chromosomes, the number of SAP gene sites on ninth chromosome has 7 genetic locus, the genetic locus in the 1th and 4th chromosome was the fewest. In all SAP stress-association protein members, 19 members located in the nucleus, 3 members located in extracellular, 5 members located in both nucleus and mitochondria; the family members have 1-4 exons; according to the different conserved domains, it will be pided into three categories, the first category has 4 members, which have RAMP4 domain, the second only has 1 member, with the zfA20 domain, the third type have 22 members, with both ZnF-AN1 domain and zfA20 domain. Evolutionary analysis showed that the SAP protein had many mutation sites, and the difference of multiple sequence alignment was larger, and the gene mutation was prone to adapt to the stress environment during the evolution. This research is to lay the theoretical foundation for the development of rape new varieties– resisitance to stress.