过氧化氢和水杨酸对蚕豆根尖生长过程的影响
毕业论文关键词：过氧化氢；水杨酸；微核率；根尖；蚕豆
Effect of Hydrogen Peroxide And Salicylic Acid on
Growth Process of Broad Bean Root Tips
Abstract: Broad bean root tips were selected in this experiment, the effect of different concentrations of hydrogen peroxide and salicylic acid on external morphology, protein content, sugar content of broad bean root tips and root tip cells micronucleus rate was researched. The results showed that a certain concentration of hydrogen peroxide (0.2%) and salicylic acid (0.4mmol/l) could promote growth of broad bean, reduce cell micronucleus rate and increase the content of sugar and protein. But when the concentration of hydrogen peroxide and salicylic acid were higher than 0.2% and 0.4mmol/L respectively, the growth of aboveground and underground parts of broad bean seedling were inhibited, specific performance was the following: height of seedling was reduced, root length was shorter, external morphology of root was abnormal. The above showed that the growth of broad bean seedling and root growth process suffered different influences under hydrogen peroxide and salicylic acid.
Key words: Hydrogen peroxide；Salicylic acid；Micronucleus rate；Root tip；Broad bean
目 录
摘 要 1
引言 2
1. 材料与方法 2
1.1 材料 2
1.2 器具、药品与试剂 2
1.3 方法 2
1.3.1 过氧化氢和水杨酸溶液的配制 2
1.3.2 种子处理 2
1.3.3 各种指标测定 3
2. 结果与分析 4
2.1 过氧化氢和水杨酸对蚕豆根系生长状况的影响 4
2.2 过氧化氢和水杨酸对蚕豆幼根中可溶性糖含量的影响 5
2.3 过氧化氢和水杨酸对蚕豆幼根中可溶性蛋白含量的影响 6
2.4 过氧化氢和水杨酸对蚕豆根尖细胞微核率的影响 6
3. 结论与讨论 7
3.1 过氧化氢和水杨酸对蚕豆根系生长状况的影响 7
3.2 过氧化氢和水杨酸对蚕豆幼根中可溶性糖含量的影响 7
3.3 过氧化氢和水杨酸对蚕豆幼根中可溶性蛋白含量的影响 7
3.4 过氧化氢和水杨酸对蚕豆根尖细胞微核率的影响 8
参考文献 8
致谢 11
过氧化氢和水杨酸对蚕豆根尖生长过程的影响引言
随着社会的发展，过氧化氢和水杨酸广泛的应用于各种领域，其在化妆品、医药行业、植物的抗逆性和储藏保鲜、消毒、漂白以及其它的方面均有重要的作用。食品级的过氧化氢因为具有高效、无残留等特性而备受喜欢，越来越多的应用在乳品、饮料、蔬菜以及瓜果等的生产和加工过程中。但是滥用过氧化氢和水杨酸都会造成一定程度的伤害[1-9]。本研究以蚕豆为实验材料，以过氧化氢和水杨酸为处理剂，研究不同浓度的过氧化氢和水杨酸对蚕豆根系外部形态、根尖细胞的微核率的影响，并测定不同处理下根尖蛋白质含量、糖含量。对过氧化氢和水杨酸进行遗传毒性的初步研究，旨在为过氧化氢和水杨酸的安全使用提供科学依据，提高人类的生活质量。