复合菌剂对四环素胁迫下小麦幼苗生长的影响
毕业论文关键词：小麦幼苗；四环素胁迫；水培体系；复合菌剂；缓解效应
Effects of Compound Bacteria on the Growth of Wheat Seedlings Under Tetracycline Stress
Abstract: Using the “zhoumai18” wheat seeds as material, the effect of compound bacterial on the chlorophyll, malonaldehyde, catalase, peroxidase, polyphenol oxidase of the wheat seedlings under the different concentrations of tetracycline coercion (10 mg/kg/, 20 mg/kg, 40 mg/kg, 60 mg/kg, 80 mg/kg, 100 mg/kg) was study by using hydroponic method. The results show that tetracycline could damage the growth of wheat seedlings. It could inhibit the growth of wheat seedlings. 108cfu/mL compound bacterial could enhance markedly the growth index of wheat seedlings under tetracycline stress, such as plant height, root length, dry weight, wet weight and chlorophyllit, photosynthetic rate. The compound bacteria could activate antioxidant enzyme activities of wheat seedlings, enhance peroxidase and catalase activities of wheat seedlings, decrease malonaldehyde concent, maintain the stability of plant cell membrane, and reduce the injuryn degree on membrane lipid peroxidation by tetracycline stress. Thus, compound bacterial alleviated the injury caused by achromycin stress on seeding growth.
Keywords: wheat seedlings; tetracycline stress; hydroponic culture; compound bacteria; mitigative effect
目 录
摘要 1
引言 1
1 材料和方法 2
1.1 试验材料 2
1.2 培养方法及处理 2
1.3 测定项目和方法 3
1.4 数据分析 3
2 结果与分析 3
2.1 复合菌剂对四环素胁迫下小麦幼苗形态指标的影响 3
2.2 复合菌剂对四环素胁迫下小麦幼苗光和参数的影响 5
2.3 复合菌剂对四环素胁迫下小麦幼苗生理指标的影响 6
2.3.1 复合菌剂对四环素胁迫下小麦幼苗叶绿素含量的影响 6
2.3.2复合菌剂对四环素胁迫下小麦幼苗过氧化物酶活力的影响 6
2.3.3复合菌剂对四环素胁迫下小麦幼苗过氧化氢酶活力的影响 7
2.3.4复合菌剂对四环素胁迫下小麦幼苗丙二醛含量的影响 8
2.3.5复合菌剂对四环素胁迫下小麦幼苗多酚氧化酶活力的影响 8
3 讨论 9
4 结论 10
参考文献 11
致 谢 13
复合菌剂对四环素胁迫下小麦幼苗生长的影响 引言
四环素类抗生素作为人畜共用的广谱性抗生素，在世界范围内使用量巨大[1-2]。由于四环素类抗生素长期大量的施用，在环境中及植物体内产生大量残留[3]，将影响植物的产量及品质和人类的健康。因此，近年来四环素类抗生素对环境及植物的影响引起了人们的关注。但是，关于缓解四环素类抗生素毒害植物的报道较少。