紫甘薯花青素抑制内皮细胞老化的研究
毕业论文关键词：PSPC 内皮细胞 高糖 细胞老化 炎性因子Purple Sweet Potato Anthocyanins InhibitEndothelial Aging Abstract As the biggest impediment to human health, cardiovascular disease prevention imminent increasingly. Since endothelial senescence is significantly related to cardiovascular disease, we try to find a breakthrough by exploring the inhibitory effect of purple sweet potato color（PSPC）on senescence of endothelial cells. In this study, human umbilical vein endothelial cells in logarithmic phase were pided into three groups for 24h and 48h :normal group, high glucose group and PSPC group ;senescence of endothelial cells was identified by β-Gal staining ;secretion of inflammatory cytokines was detected by enzyme-linked immunosorbent assay（ELISA）; NO expression was tested by NO kit; cell cycle was analyzed by flow cytometry . The result of β-Gal staining showed PSPC suppressed high glucose-induced endothelial cell aging .Meanwhile, PSPC inhibit the secretion of inflammatory cytokines in endothelial cells as showed by ELISA test, but endothelial function was restored by PSPC via increasing NO concentration. Therefore, we got the conclusions : PSPC inhibited high glucose-induced senescence of endothelial cells, which may be related with cell cycle, inhibition of inflammatory cytokine secretion and restore cell function.
Key words: PSPC Endothelial cells high glucose senescence inflammatory cytokines
目录
摘要....IV
Abstract..V
目录VI
1.引言....1
1.1心血管疾病.1
1.2内皮细胞与心血管疾病.....1
1.3糖尿病与心血管相关疾病...1
1.4紫甘薯花青素......1
2．实验材料与仪器..2
2.1实验材料和试剂....2
2.2仪器设备...3
3．实验方法3
3.1试剂配制...3
3.2内皮细胞的复苏和传代培养.5
3.3内皮细胞的分组给药......5
3.4β-Gal染色内皮细胞......5
3.5IL-8酶联免疫吸附测定试剂盒检测IL-8...6
3.6MCP-1酶联免疫吸附测定试剂盒检测MCP-1.7
3.7NO测试盒检测内皮细胞中NO浓度..8
3.8单染法检测内皮细胞周期..9
4．实验结果与分析..9
4.1β-Gal染色内皮细胞......10
4.2紫甘薯花青素抑制内皮细胞的炎性因子分泌10
4.3紫甘薯花青素恢复内皮细胞功能...11
4.4紫甘薯花青素逆转细胞周期的阻滞.12
5.讨论.12
5.1创新点....13
5.2问题......13