摘要：银杏叶中含有大量的黄酮类化合物，但是溶解度差、生物利用率低等缺点限制了其在药物、食品、化妆品等领域的应用。通过生物转化可以改变其结构，可改善其溶解性，增强其生理活性及稳定性。本文详细介绍了银杏黄酮的生物转化，并对生物转化银杏黄酮的发展前景进行了分析。还利用微生物转化对银杏黄酮进行了生物转化，研究发现利用灵芝真菌对银杏黄酮产生了转化作用，在银杏叶粉末转化液和银杏黄酮转化液中总黄酮分别降低了61.1%和99.29%，体外生物活性分别提高了2.3%和5.7%。57433

毕业论文关键词：灵芝；银杏黄酮；生物转化；微生物转化；生物活性 

Abstract: Ginkgo leaves contain large amounts of flavonoids, but poor solubility, bioavailability and low defects limits its application in pharmaceutical, food, cosmetics and other fields. Biotransformation can change its structure to improve its solubility, enhancing stability and physiological activity. This paper describes the biotransformation of Ginkgetin, and biotransformation prospects flavonoids were analyzed. Also the use of microbial transformation of flavonoids were biotransformation, the study found the use of Ganoderma lucidum fungi to produce a transformation of flavonoids in ginkgo leaf powder and liquid conversion conversion solution flavonoids total flavonoids were reduced 61.1%和99.29%, in vitro biological activity increased by 2.3% and 5.7%.

Keywords:Ganoderma lucidum;Ginkgetin; Bioconversion; Microbial transformation;

Mioactivity 

目录

1. 前言 4

1.1本课题的研究意义 4

1.2 我国银杏叶资源 4

1.3银杏叶中的成分 4

1.4 银杏黄酮的生理作用 5

1.5 银杏黄酮的应用 5

1.6生物转化的反应类型 5

1.7 本课题的发展前景 6

1.8 论文设计思路 6

2.材料与方法 7

2.1实验材料 7

2.1.1菌种 7

2.1.2原料与试剂 7

2.1.3仪器 7

2.1.4培养基的配置 8

2.1.5实验试剂的配置 8

2.2实验方法 8

2.2.1菌种的活化 8

2.2.2转化培养 8

2.2.3转化液中黄酮的测定 9

2.2.4 转化液生物活性的测定 9

2.2.5紫外吸收光谱的测定 10

3.结果与分析 11

3.1转化液中的黄酮含量 11

3.1.1 标准曲线的绘制 11

3.1.2转化液中银杏总黄酮 11

3.2羟自由基清除率 12

3.3紫外吸收光谱图 13

4.结论与展望 15

4.1 结论 15

4.2 展望 15

参考文献 16

致  谢