苯嘧磺草胺对野生大豆生长影响及噻二唑衍生物的作用研究
The Studies of Saflufenacil Effect on Wild Soybean Seedlings and the Role of 1,3,4-Thiadiazole Derivative
Abstract：Using wild soybeans “ZS” as experiment material, effects of the different concentration (0, 5, 10 and 15mg/L) of N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole -2- yl]-N '- 4-Nitrobenzoyl Urea (TNU) on the growth of wild soybean seedling under saflufenacil stress was studied by sand culture. The results showed as follow: （1）under saflufenacil stress, the height of seedling and fresh weight were significantly decreased, while the content of chlorophyll, soluble proteins and soluble sugar were significantly decreased than those of control. The contents of proline and MDA were significantly increased(P<0.01).(2) Exogenous N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole-2-yl]-N'- 4-Nitrobenzoyl Urea (TNU) application on the leaf significantly increased the height of seedling and fresh weight, and the contents of chlorophyll, soluble proteinsand proline were significantly increased(P<0.01). The content of malonaldehyde (produce of membrane lipid peroxidation) was significantly decreased(P<0.01). The results showed that exogenous N- [5- (4- Chlorophenyl ) -1,3,4- Thiadiazole -2- yl]-N '- 4-Nitrobenzoyl Urea (TNU) could effectively mitigate the harmful effects from saflufenacil stress on plants, and promote the growth of wild soybeans, and the effect of 10mg/L TNU was optimal.
Key Words：1,3,4-thiadiazole ; Saflufenacil ; Soybean ; Physiological characteristics
目 录
摘要 1
Abstract 1
引言 2
1材料与方法 2
1.1 试验材料 2
1.2试验方法 3
1.2.1材料处理 3
1.2.2 测定项目与方法 3
1.3数据分析 4
2结果与分析 5
2.1 TNU对苯嘧磺草胺胁迫下野生大豆幼苗苗高的影响 6
2.2 TNU对苯嘧磺草胺胁迫下野生大豆幼苗鲜重的影响 7
2.3 TNU对苯嘧磺草胺胁迫下野生大豆幼苗叶片内叶绿素含量的影响 7
2.4 TNU对苯嘧磺草胺胁迫下野生大豆幼苗叶片内可溶性蛋白含量的影响 8
2.5 TNU对苯嘧磺草胺胁迫下野生大豆幼苗叶片内脯氨酸含量的影响 9
2.6 TNU对苯嘧磺草胺胁迫下野生大豆幼苗叶片内MDA含量的影响 10
3讨论 10
参考文献 11
致谢 12
苯嘧磺草胺对野生大豆生长影响及噻二唑衍生物的作用研究引言
大豆作为一种起源于中国的农作物，它具有生物体所需要的优质蛋白质和食用油脂等营养物质，在粮食生产中占有极为重要的地位。但近年来栽培大豆研究遇到瓶颈，原因是大豆亲本遗传基础狭窄，缺乏丰富的基因源。如今农业上用作栽培的大豆的原始祖先种是一年生的野生大豆。它是一年生豆科草本植物，具有蛋白质含量高，抗逆性强和繁殖系数大等特点。喷洒除草剂是大豆的生产过程中避免的环节，本实验使用的苯嘧磺草胺是一个新的嘧啶二酮类除草剂。主要用于大豆、玉米、棉花、向日葵等多种作物的播前或出苗前进行除草处理。但近些年来由于滥用除草剂导致的烧苗、减产、影响下茬作物以及环境污染等问题屡见不鲜。针对此现象，科学家进行了许多科学探究实验。最终通过化学方法合成了1,3,4-噻二唑衍生物，它具有较好的杀虫、抗病菌、除草以及植物生长调节等功效[3-10]。因此本实验通过运用不同浓度的噻二唑衍生物对被除草剂处理过的野生大豆幼苗生长的影响，对比各个浓度下同一生长时期野生大豆幼苗的根长、幼苗的鲜重、叶片中叶绿素含量、叶片中可溶性蛋白含量、游离脯氨酸含量和丙二醛含量等指标，从而为被除草剂处理过的野生大豆幼苗再生长找到该衍生物的最适浓度。为大豆生产找到最合适的生长调节剂及合理施用方面提供科学的理论依据。