人SEPT 9蛋白序列特性和结构建模研究
关键词：人；SEPT 9蛋白；模建；活性位点；生物信息学
The Study of sequence characters and homology modeling
of human SEPT 9 protein
Abstract: SEPT9 protein widely exists in eukaryotic organism , members of the septin superfamily, GTP-binding protein, which is related to cell pision cycle. SEPT9 protein plays an important role in many aspects such as cell pision, material transportation and human diseases, such as brain tumors, colorectal cancer, breast cancer as well as nervous system degenerative diseases such as Parkison disease and Alzheimer's disease. In this paper, SEPT9 proteins were classified into three classes on the bases of analysis on multiple sequence alignment by using bioinformatics methods. And physical and chemical properties, structures, the evaluation of its tertiary structure results, active site are analyzed and predicted. The results show that the structure of SEPT9 protein and its family members is conservative, they have smaller difference in amio acid composition and sequence length ,globular proteins are unstable,no transmembrane and signal peptide; Secondary structure is given priority to alpha helix and random coil. The tertiary structure of SEPT9 protein (XP_006721706.1) was gotten, which meets the rule of stereochemistry in the figure of Ramachandran, There are lots of ligands binding sites ,102 sites may be the active site of the protein.
Keywords:human being；septin9 protein；modeling；active site; bioinformatics
目 录
摘 要 1
引 言 2
1 材料和方法 4
1.1 数据来源 4
1.2 人SEPT 9蛋白同源性比较、聚类分析及理化性质分析 4
1.3 磷酸化位点、信号肽预测、和亚细胞定位分析 4
1.4 跨膜区、紊乱区和球蛋白区预测 4
1.5 SEPT 9蛋白的进化分析 4
1.6 二级结构、三级结构预测及建模结构的评估 4
1.7 活性位点信息分析 5
2 结果与分析 5
2.1 人SEPT 9蛋白同源性比较及聚类分析 5
2.2人SEPT 9蛋白理化性质分析 7
2.3磷酸化位点、信号肽预测、亚细胞定位和前导肽分析 8
2.4 人SEPT 9蛋白跨膜区分析 9
2.5紊乱区、球蛋白区预测 10
2.6 SEPT 9蛋白垂直同源序列的比较和进化树构建 11
2.7 二级结构预测、三级结构的预测及建模结构的评估 13
2.7.1 二级结构预测 13
2.7.2 结构的模建与评价 13
2.8活性位点信息分析 16
3讨论 17
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