(3) The inhibition of ethyl acetate, n-butanol and water phases of the ethanol extracts from pomegranate peel on Macrococcus caseolyticus and Micrococcus luteus was further examined, and the results showed that the n-butanol phases had strong inhibition on the two tested microorganisms. The MIC value of pomegranate peel  n-butanol phases to Macrococcus caseolyticus and Micrococcus luteus were 12.5 mg/mL, which were similar to the ethanol extracts from pomegranate peel; the MIC values of pomegranate peel ethyl acetate phases were 50 mg/mL and 25 mg/mL to Macrococcus caseolyticus and Micrococcus luteus respectively, which were higher than the ethanol extracts from pomegranate peel. While the water phases had no antibacterial activity to Macrococcus caseolyticus and Micrococcus luteus. The result showed that the main antimicrobial active substances of the ethanol extracts from pomegranate peel were in n-butanol phases.

(4) The effects of heat treatment and UV irradiation on antibacterial activity of pomegranate peel n-butanol phases were examined. The result showed that the antibacterial activity to Macrococcus caseolyticus was stable to high temperature and UV irradiation.

毕业论文关键词：石榴果皮；抑菌活性；正丁醇相

Keyword: pomrgranate peel;inhibition activity;n-butanol phases

目录 4

引言 5

1 材料与方法 5

1.1试材 5

       1.1.1 供试植物样品 5

       1.1.2 供试菌种 6

       1.1.3 培养基 6

1.2 方法 6

       1.2.1 石榴果皮的溶剂提取 6

       1.2.2 培养基及含药培养基的制备 6

       1.2.3 供试菌株的准备 7

       1.2.4 石榴果皮乙醇提取物最低抑制浓度（MIC）的测定 7

       1.2.5 石榴果皮乙醇提取物抑菌活性测定 7

       1.2.6 石榴果皮提取及分级萃取 7

       1.2.7 石榴果皮萃取物抑菌活性测定 8

       1.2.8 热处理对石榴果皮正丁醇萃取物抑菌活性的影响 8

       1.2.9 紫外线照射对石榴果皮正丁醇萃取物抑菌活性的影响 8

2 结果与分析 8

2.1 石榴果皮乙醇提取物抑菌活性测定结果 8

2.2 石榴果皮乙醇提取物 MIC 测定结果 10

2.3 石榴果皮提取及分级萃取结果 10

2.4 石榴果皮萃取物抑菌活性测定结果 10

2.5 热处理对石榴果皮正丁醇萃取物抑菌活性的影响 12

    2.6 紫外线照射对石榴果皮正丁醇萃取物抑菌活性的影响 12

3 讨论 13

3.1 研究意义 13

3.2 石榴果皮乙醇提取物对常见肉质腐败菌的影响 13