摘要本试验以产自海南的火龙果果皮为原料，研究了以纯水、95％乙醇、乙酸乙酯、正己烷为溶剂的火龙果皮提取液的抑菌活性，选取了四个常见的食品致病菌进行试验研究：金黄色葡萄球菌（革兰氏阳性菌）、枯草芽孢杆菌（革兰氏阳性菌）、大肠杆菌（革兰氏阴性菌）、白色念珠菌（真菌）。76615

    实验结果表明，火龙果皮的水、95％乙醇、乙酸乙酯、正己烷提取液对细菌、真菌都有一定的抑制效果。水提取物对金黄色葡萄球菌的抑制作用更显著，抑菌率为（12。0%±3。0%）；乙醇提取物、正己烷提取物均对大肠杆菌有显著的抑制作用，相比而言，正己烷提取物的抑菌作用更显著，抑菌率为36。9%；乙酸乙酯提取物、正己烷提取物在最大浓度（10mg/ml）时对白色念珠菌均有显著的抑菌作用。通过高效液相色谱分析可知，水提取物中含有咖啡酸、表儿茶素和 4-羟基桂皮酸；乙醇提取物中含有绿原酸、p-香豆酸、根皮苷；乙酸乙酯提取物中含有丁香苷、原花青素B2；正己烷提取物中含有丁香苷、槲皮素。

Abstract  Pitaya Peel(from Hainan) was chosen as materials of study in this research。Extract of Pitaya Peel by water, 95% ethanol, ethyl acetate and n-hexane was tested for its antimicrobial activity。Four soecies of common food contaminating microorganisms were used astested microorganism: Staphylococcus aureus (gram-positive bacteria), Bacillus subtilis (gram-positive bacteria), Escherichia coli (gram negative bacteria), Candida albicans (fungus)。

    The experimental results show that: extract of Pitaya peel had inhibitory effect on bacteria, fungi。Extract of water whose inhibition rate was(12%±3%) had more sighificant inhibitory effecct to staphylococcus aureus。 inhibition rate。 Ethanol extract and n-hexane extract have significant inhibitory effect on escherichia coli,and the bacteriostatic action of n-hexane extracts which was 36。9% was more prominent。 Ethyl acetate extracts and hexane extracts at the maximum concentration (10mg / ml), the Candida albicans were significantly inhibited。 Analysis by HPLC shows that water extract contains caffeic acid, epicatechin and 4-hydroxy cinnamic acid。 Ethanol extract contains chlorogenic acid, p-coumaric and phlorizin。 Ethyl acetate extract contains syringin and procyanidin B2。 Hexane extract contains cloves glycosides and quercetin。

毕业论文关键词：火龙果果皮； 提取物； 多酚； 抑菌活性

Keyword:  Pitaya Peel;  Extract;  Polyphenol;  Antibacterial activity

目录

目录 3

引言 4

1 材料与方法 5

1。1  供试材料 5

1。1。1 供试样品 5

1。1。2 供试菌种 5

1。1。3 培养基 5

1。2  试验方法 6

1。2。1总酚含量的测定 6

1。2。1。1样品的处理 6

1。2。1。2标准品溶液的制备并绘标准曲线 6

1。2。1。3样品溶液的配制 6

1。2。2火龙果皮不同极性溶剂的提取 6

1。2。3火龙果皮提取物抑菌活性测定 7

1。2。3。1 牛津杯法 7

1。2。3。2 微孔板法 7

1。2。4火龙果皮提取物的高效液相色谱分析