摘要本文以白藜芦醇为研究对象，随着经济的发达，人们对饮食的要求越来越高了，但是有些不合理的加工或生产，给消费者带来了极大的伤害及疾病。比如丙烯醛中毒。丙烯醛是一种强刺激性挥发性气体，人体经消化系统，呼吸系统，皮肤黏膜等多种方式接触。吸入丙烯醛轻易引发流泪，眼痛，头晕，头疼，呼吸困难等症状。白藜芦醇是一种自然的多酚类化合物，来自于花生等植物。是一种生抗氧化性很强的天然多酚类物质，又被称为芪三酚，是肿瘤的预防剂，也是对降低血聚集，预防和治疗动脉粥样硬化、心脑血管等疾病的化学预防剂。本实验研究天然植物多酚白藜芦醇降低丙烯醛毒性效果。本实验利用白藜芦醇进行研究抑制丙烯醛毒性。87956

In this paper, resveratrol as the research object, with the development of economy, people's dietary requirements are getting higher and higher, but some unreasonable processing or production, to the consumer has brought great harm and disease。 Acrolein poisoning。 Acrolein is a strong irritant volatile gases, the human body through the digestive system, respiratory system, skin mucosa and other ways to contact。 Inhalation of acrolein can cause tears, eye pain, dizziness, headache, difficulty breathing and other symptoms。 Resveratrol is a natural polyphenolic compound, which is derived from peanut, grape (red wine), Polygonum cuspidatum, mulberry and other plants。 Resveratrol is a kind of biological strong natural polyphenols, also known as Qi three phenol, is the chemical agents for the prevention of cancer, but also to reduce platelet aggregation, chemopreventive agents for prevention and treatment of atherosclerosis, cardiovascular and cerebrovascular diseases。 This study was conducted to study the effect of natural plant polyphenol resveratrol on reducing acrolein toxicity。 In this study, resveratrol was used to study the inhibition of acrolein toxicity源-于,优Y尔O论U文.网wwW.youeRw.com 原文+QQ75201,8766

毕业论文关键词：丙烯醛 白藜芦醇 蛋白毒性 抗氧化性

Key words: Acrolein ；Resveratrol ；Protein toxicity；Oxidation resistance

目录

1。 引言 4

2。 材料和方法 4

2。1材料与设备 4

3。 高效液相色谱（HPLC）法 4

3。1实验步骤 4

3。2 实验方法 5

4。 SDS-PAGE电泳法与western blot法 6

4。1实验步骤与结果 6

4。2 DNPH衍生法测ALEs 7

4。3  RES的检测 8

4。4 实验结果与分析图 9

 参考文献 11

 致谢 11

1。引言

    ACR是丙烯醛，没有颜色、是有毒、有猛烈刺激性气味的液体，与H2O部分互溶。其蒸气有强烈催泪性。主要由丙烯氧化而。人体可通过消化系统，呼吸系统，皮肤黏膜等多种方式接触。吸入丙烯醛轻易引发流泪，眼痛，头晕，头疼，呼吸困难等症状。因此在日常生活中，烟气，汽车尾气，油烟，灸烤食品等均有大量的丙烯醛。丙烯醛按输入的量不同，对人体造成的危害也有差别，少剂量的丙烯醛能够抑制细胞增值从而增加细胞凋亡，而多剂量的会引发肿瘤。丙烯醛对四大系统造成风险。From~优Y尔R论^文W网wWw.YoUeRw.com 加QQ7520.18766