摘要：为有效地提取分离紫薯汁中的花青素，采用反相乳液法制备多种壳聚糖树脂并找到最佳壳聚糖树脂，采用90%甲醇和90%乙醇溶液对紫薯汁中的花青素进行分离提取。在制备的壳聚糖树脂对紫薯汁中花青素吸附特性研究的基础上，采用壳聚糖树脂吸附-柱层析分离法，对紫薯汁中花青素进行解吸。结果显示，最佳壳聚糖树脂是加入的戊二醛的量为体系的10%,最佳pH为3。0,静态平衡吸附量为36。53mg/g。在动态吸附中，当选取最佳壳聚糖,pH为3。0，紫薯汁初始糖度为4°Brix，流速为0。5mL/min时，壳聚糖树脂对紫薯汁中花青素的吸附效率达到最大。紫薯汁中花青素经最佳壳聚糖树脂吸附后，用90%甲醇和90%乙醇以不同的流速分别洗脱，当洗脱剂为90%甲醇，流速为0。5mL/min时解吸效率最佳，解吸率为80。1%。79904

毕业论文关键词：壳聚糖树脂、紫薯汁花青素、吸附特性、吸附、解吸

Adsorption and separation of anthocyanins from purple sweet potato juice by chitosan resin

Abstract: In order to effective extraction of anthocyanins from purple sweet potato juice, this paper to produce a variety of chitosan resin by inverse emulsion polymerization, and find the optimal chitosan resin to separation and extraction of anthocyanins in purple sweet potato jui-

ce。90% methanol and 90% ethanol can help to extract and separate anthocyanins of purple s- weet potato juice。 On a basis of the study, which finds chitosan resin has adsorption on anth-

ocyanins of purple sweet potato juice, chitosan resin adsorption column chromatography can be a way to desorb anthocyanins of purple sweet potato juice。 The result shows that the opti- mal chitosan resin can be derived when added glutaraldehyde is 10% of the system, the best pH is 3。0, and the amount of static equilibrium adsorption is 36。53mg/g。 In the dynamic adso- rption, when choosing the optimal chitosan resin, making sure the pH is 3。0, the initial sugar content of purple sweet potato juice is 4°Brix, and the flow rate is 0。5mL/min, the chitosan resin will have the maximum adsorption efficiency of anthocyanins of purple sweet potato juice。 After the optimal chitosan resin adsorption on anthocyanins of purple sweet potato juice, elution with 90% methanol and 90% ethanol in different loading rate, the best desorption effi- cciency will achieve when eluent is 90% methanol, the flow rate at 0。5mL/min, and the de-

sorption rate will be 80。1%。

Keywords:Chitosan resin; Purple sweet potato juice; adsorption characteristics; adsorption; desorption

目录

1引言 1

2材料与方法 2

2。1 材料与试剂 2

2。2 仪器与设备 2

2。3 试验方法 3

2。3。1 壳聚糖树脂的制备 3

2。3。2 吸附试验 3

2。3。3 解吸试验 4

2。3。4 数据分析 4

3 结果与分析 5

3。1 壳聚糖树脂对紫薯汁中花青素的静态吸附 5

3。1。1 最佳pH 5

3。1。2 吸附时间动力学 6

3。2 壳聚糖树脂对紫薯汁花青素的动态吸附 6

3。3紫薯汁中花青素的动态洗脱 9

致  谢 10

参考文献