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摘要：目的：本文旨在研究曲克芦丁对肺腺癌A549细胞的抑制作用并初步探讨其分子机制。方法：A549细胞复苏与传代；划痕实验，研究细胞在不同溶度的曲克芦丁中的迁移情况；收集曲克芦丁处理后的细胞培养液上清检测清除自由基水平；流式细胞仪检测细胞周期实验。
结果：在倒置显微镜下观察曲克芦丁处理过的细胞，发现增殖速度受到明显抑制；划痕实验中A549细胞经曲克芦丁处理后，24h,48h后增殖、迁移速度随化合物浓度增加而降低；在细胞培养液上清清除自由基水平实验中，酶标仪检测出不同浓度曲克芦丁培养液上清液吸光度，来计算出清除率，得出浓度升高清除率升高；流式细胞仪检测细胞周期结果显示，200 uM的曲克芦丁可以将细胞周期阻滞在G1期。51847
结论：曲克芦丁能够通过靶向A549细胞的G1期，抑制肿瘤细胞的增殖，这种作用可能与其清除自由基和抗氧化活性相关。
毕业论文关键词肺癌A549细胞 细胞增殖 细胞周期 曲克芦丁
Inhibitory role of troxerutin on human lung cancer A549 cells
Objective: This paper aims to investigate the inhibitory effect of troxerutin on human lung cancer A549 cell and to make clear the possible mechanisms.
Methods: Thaw and passage the A549 cells; Wound scratch test was performed to test cell migration after troxerutin treatment with different concentration; Supernatant in different groups were collected to detect anti-oxidation activity; Flow cytometry was performed to identify cell cycle.
Results: Under inverted microscope, we found troxerutin significantly inhibited proliferation of A549 cells; The migration of A549 was suppressed by troxerutin in dose-dependent manner as shown in wound scratch test; The result of anti-oxidation experiments showed that troxerutin could eliminate free radicals dramatically in comparison with normal A549 cells; Meanwhile, the analysis of flow cytometry suggested that cell cycle of A549 cells was blocked in G1 phase after administration of 200 uM troxerutin .
Conclusion:Troxerutin can inhibit the proliferation of A549 cells by targeting G1 phase, and the activity of anti-oxidation might be involved in this process.
Key words
Lung cancer A549 cell cellular proliferation cell cycle ntroxerutin
目录
1.引言 4
1.1 肺癌 5
1.2 A549细胞 5
1.3 细胞增殖 5
1.4 黄酮类化合物 曲克芦丁 5
1.5 氧化压力与肿瘤 5
1.6 细胞周期 6
2.材料与方法 6
2.1 材料
2.1.1 器材 6
2.1.2 材料 6
2.1.3 试剂 7
2.1.4 黄酮类化合物（T） 8
2.2方法
2.2.1 A549细胞复苏及培养 8
2.2.2 A549细胞的传代 8
2.2.3 A549细胞培养液的更换 9
2.2.4 A549细胞的冻存 9
2.2.5 曲克芦丁的处理