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摘 要：杜仲具有多种保健功能，本文从抗氧化角度出发，以杜仲根部、木质部为原料，采用连续光谱酶标测试仪分别测定其粗提物各相萃取物的清除DPPH自由基活性、还原能力和金属离子螯合能力，并选用市场上常见的抗氧化剂BHA、BHT、THBQ、VC进行比较。实验发现：清除DPPH自由基活性时，除杜仲根水溶相提取物及杜仲木质部正己烷相萃取物较BHA抗氧化效果稍差，其余萃取相提取物效果都显著高于BHA；还原能力测定中，结果大体符合清除DPPH自由基测定结果；但在金属离子螯合能力测定中，所有样品的螯合能力都比较低；结果表明石油醚、二氯甲烷和乙酸乙酯等有机溶剂可以用于富集杜仲根、杜仲木质部的抗氧化成分，杜仲根及木质部可以在杜仲叶、杜仲花之后成为新开发的抗氧化剂原料。54400
毕业论文关键词：杜仲；杜仲木质部；抗氧化活性
Abstract: Eucommia ulmodies Olive has a variety of health care function. Roots and wood of E.ulmodies were used as raw material in this work based on the perspective of antioxidant way. We used continuous spectrum enzyme mark tester to measure the removal activity of DPPH free radicals, reducing power and metal ion chelating ability of its crude extract of each fraction respectively, meanwhile BHA, BHT, THBQ, VC were used as positive control. It was found that when we measured the removal activity of DPPH free radicals, most of the extraction phase extracts had better antioxidant effect than the BHA escape for the water soluble phase extract of roots and the n-hexane extracts of wood. The result of measuring reducing power was mostly accorded with what we got from measuring the removal activity of DPPH free radicals. When we measured the metal ion chelating ability, we found out that the chelating ability of all of the fractions were bad. The result of this experiment turns out that those organic solvents such as the petroleum ether, the methylene chloride and the ethly acetate can be used to gather the antioxidant ingredient of roots and wood of E.ulmodies. Roots and wood of E.ulmodies can be made into new antioxidant ingredients after the exploitation of leaves and flowers.
Key word: Eucommia ulmodies；Wood of E. ulmoides ; Antioxidant activity
目录
1 前言 5
1.1 杜仲简介 5
1.2杜仲功效 5
1.3抗氧化活性简介 6
2 材料与方法 7
2.1 试剂与仪器 7
2.1.1 试剂 7
2.1.2 仪器 7
2.2 实验方法 8
2.2.1 杜仲根、木质部粗提物及各萃取物的准备 8
2.2.2 清除DPPH 自由基活性测定 8
2.2.3 还原能力测定 8
2.2.4 金属离子螯合能力测定 8
2.2.5 数据处理 9
3 结果与分析 9
3.1 杜仲粗提物及各相萃取物的分析 9
3.2 杜仲粗提物及各相萃取物清除DPPH自由基 9
3.3 杜仲粗提物及各相萃取物还原能力 11
3.4 杜仲粗提物及各取萃取物金属离子螯合能力 12
结论 13
参考文献 14
致谢