蛋白药物在PLGA微球中的包埋与缓释研究

摘要PLGA微球是一种可生物降解的聚合物载体，普遍应用于在体内稳定持续的释放多肽/蛋白药物，与生物体的相容性高。本文采用乳液挥发法制备了一种可生物降解的PLGA微球，以牛血清蛋白（BSA）为模型药物,体外模拟PLGA包药微球的药物释放和降解过程。结合表面接枝改性技术，以戊二醛为交联剂，将明胶蛋白分子接枝于PLGA微球表面。通过SEM、红外光谱仪、紫外分光光度计等对PLGA微球的形貌、载药量、粒径大小、降解速率、释放过程、红外光谱等特性进行表征。结果表明，制得的PLGA包药微球形状规则，表面圆整，平均粒径约为93μm，包封率可达75。99%，在两周的时间内可以持续稳定地释放药物，累计释放比超过93%，同时还具备良好的生物降解性能。微球表面进一步接枝明胶分子后，蛋白含量增加了3。8%，有效提高了PLGA微球材料的生物活性。87686

毕业论文关键词 PLGA微球蛋白药物释放降解

毕业设计说明书外文摘要

Title Research on protein drugs embedding and releasing of poly(lactic-co-glycolic acid, PLGA) microspheres

Abstract PLGA microspheres is a kind of drug delivery carrier which has good biocompatibility and controlled biodegradability。 Compared with other microspheres, the body produces less immune response by using PLGA microspheres。 In this study, a biodegradable PLGA microspheres were prepared by solvent evaporation method。 The release and degradation process of PLGA microspheres was simulate d in vitro using bovine serum albumin (BSA) as drug model。 Combined with surface grafting modification technology, the gelatin protein molecules were grafted onto the surface of the microspheres with glutaraldehyde as the crosslinking agent。 Properties of PLGA microspheres such as drug loading, particle size distribution, microstructure, degradation rate, release process were characterized by SEM, infrared spectrometer, UV spectrophotometer。 The results showed that the prepared PLGA microspheres were in regular circle。 The average particle size is about 93μm and the encapsulation efficiency reached 75。99%。 The microspheres can release drug continuously and stably in two weeks and the cumulative release ratio reached 93%。 Moreover, it has good biodegradability。 After grafting gelatin molecules onto the surface of the microspheres, the content of protein was increased by 3。8%, which effectively improved the biological activity of PLGA microspheres。

Keywords PLGA microspheres protein drugs release degradation

1 绪论 1

1。1 组织工程 1

1。2 医用高分子材料 2

1。3 PLGA微球 3

1。4 药物包埋及释放 5

1。5 骨组织工程 7

1。6 课题的提出及内容 8

2 微球的制备和表征 9

2。1 实验材料及设备 9

2。2 实验方法 10From+优|尔-论_文W网wWw.YouErw.com 加QQ752018.766

3 实验结果与分析 14

3。1 微球的SEM图 14

3。2 微球的平均粒径及粒度分布 15

3。3 PLGA包药微球的载药量和包封率 15

3。4 PLGA微球的体外释放蛋白药物在PLGA微球中的包埋与缓释研究:http://www.youerw.com/yixue/lunwen_143191.html