磁性介孔SiO2微球的制备及性能研究
毕业论文关键词：磁性/介孔纳米粒子；二氧化硅；共沉淀法；细胞毒性；蛋白吸附
Preparation and Properties of Mesoporous SiO2 Magnetic Microspheres
Abstract：With the development of Nano-biotechnology, the magnetic Nano-Fe3O4 plays an important role in the field of mechanical, biomedical and other fields. In this paper, co-precipitation is chosen to synthetize Fe3O4.Then the Fe3O4 is wrapped in mesoporous silica by one-step method. Magnetic microspheres with high magnetic saturation, magnetic saturation, and high specific surface were prepared. SEM, TEM, size test, BET and other tests are used to compare the magnetic microspheres made by different amounts of ammonia. At the same time, the influences of the ammonia amount in the magnetic microspheres to the cell’s biological safety and adsorption of protein were analyzed with the MCF-7 as model cell and HAS as model protein. Then, the drug loaded and the drug release behavior were also characteristic.The results show that, with the absence of Fe3O4 nanoparticles, the mesoporous silica microspheres are spherical with high specific surface area, high pore volume and pore characteristics, as well as high saturated magnetic intensity and excellent magnetic response function. Because of its good biological safety, this material could be used in the drug delivery system in the future.
Key word：Mesoporous magnetic particles; SiO2, co-precipitation; biological safety; Protein Adsorption
目 录
1.绪论 .. 1
1.1什么是介孔材料 .. 1
1.2介孔二氧化硅 2
1.2.1介孔二氧化硅的合成机理 . 2
1.2.2介孔二氧化硅制备方法 .. 2
1.3环境响应性介孔二氧化硅载体 .. 3
1.4 磁性介孔二氧化硅微球的研究进展 4
1.4.1 合成方法 . 4
1.4.2 磁性介孔二氧化硅材料的合成机理 .. 5
1.4.3磁性介孔二氧化硅应用 .. 6
1.5本课题研究的目的和意义 . 7
1.6本课题研究的主要内容 .. 7
2.实验部分 9
2.1实验原料 .. 9
2.2主要仪器和设备 .. 9
2.3实验方法 10
2.3.1 MSNs的制备 .. 10
2.3.2 Fe3O4纳米晶的制备 10
2.3.3溶胶-凝胶法制备 Fe3O4@MSNs . 11
2.3.5 MSNs及磁性响应 MSNs 微球的药物担载 . 12
2.4测试与表征 .. 12
2.4.1介孔二氧化硅磁性微球的基础理化测试 .. 12
2.4.2细胞毒性测试 . 13
2.4.3人血清蛋白（HAS）黏附测试 14