摘要：本文介绍了一种由钾掺杂碳纳米管修饰电极来固化葡萄糖氧化酶的新型葡萄糖生物传感器。钾掺杂碳纳米管加快了电子转移速率，导致了葡萄糖氧化酶的直接电化学，同时还保持了其生物活性。作为葡萄糖分析的一个新平台，钾掺杂碳纳米管修饰电极的线性范围为 0.1-3.0 mmol L−1，检测限为 0.02 mmol L−1 (S/N = 3)，对葡萄糖展现出高灵敏度。在-0.52 V的检测电位下，此生物传感器对葡萄糖受到的常见的干扰，比如抗坏血酸（AA）和尿酸（UA）的干扰等，表现出很高的灵敏度和选择性。这些结果表明,葡萄糖氧化酶/ 钾掺杂碳纳米管修饰的玻碳电极在葡萄糖检测中呈现稳定的高灵敏度，对葡萄糖检测也表现出快速测量电流的反应,充分展现了葡萄糖传感器的发展前景。71288

毕业论文关键词：钾掺杂碳纳米管，葡萄糖氧化酶，电催化，生物传感器

Abstract: A novel biosensor for glucose was prepared by immobilizing glucose oxidase (GOx) on Potassium-doped carbon nanotubes (KMWNTs) modified electrode. The KMWNTs accelerate the electron transfer from electrode surface to the immobilized GOx, leading to the direct electrochemistry of GOx and maintaining its bioactivity. As a new platform in glucose analysis, the resulting electrode (GOx/KMWNTs/GCE) exhibits a sensitive response to glucose, with a linear range form0.1 to 3.0 mmol L−1 and a detection limit of 0.02 mmol L−1 (S/N = 3). At an applied potential of -0.52 V, the resulting biosensor performs a sensitive and selective electrochemical response to glucose in the presence of common interferences, such as ascorbic acid (AA) and uric acid (UA). These results indicated that the GOx/KMWNTs modified glassy carbon electrode presents stable, high sensitivity and also exhibits fast amperometric response to the detection of glucose, which is promising for the development of glucose sensor.

Keywords: Potassium-doped carbon nanotubes; Glucose oxidase; Direct electrochemistry; Biosensor; 

目 录

1  前言 6

1.1  电化学传感器的简介 6

1.1.1电化学传感器的概述 6

1.1.2电化学传感器的种类 6

1.2碳纳米管简介 6

1.2.1碳纳米管的特性 6

1.2.2碳纳米管的制备 7

1.2.3碳纳米管的应用 7

1.3本课题主要内容及意义 8

2  实验部分 9

2.1试剂 9

2.2葡萄糖的制备 9

2.3钾掺杂碳纳米管的制备 9

2.4制备酶修饰电极 9

2.5仪器 10

3  结果与讨论 10

3.1KMWNTs电化学阻抗光谱表征 10

3.2用KMWNTs修饰的电极来固定氧化酶的直接电化学行为 11

3.3用GOx/KMWNTs修饰的玻碳电极检测葡萄糖的直接电化学行为 13

3.4生物传感器的抗干扰性和稳定性 13

结论 16

参考文献 17

致谢 18

1  前言