灵芝活性成分的提取富集工艺研究
本研究通过前阶段对沪农1号50KG灵芝子实体进行了中试提取，取部分提取物用50%，60%，70%，80%，90%，100%乙醇溶液在D-101、HZ-801、HZ-818三种大孔树脂做分段洗脱实验，发现最适合富集灵芝三萜化合物的大孔树脂为D-101型大孔树脂，同时将各分段洗脱下来的提取物进行了高效液相色谱分析和抗肿瘤活性测定。分析结果表明三萜化合物主要集中在90%和100%醇提灵芝液中，且这部分的洗脱物具有很强的细胞毒性。随后进行了大孔树脂的中试洗脱，最终获得了富含三萜化合物的组分。将这一组分进行了硅胶柱层析，获得了麦角甾醇、灵芝酮三醇两个化合物。
毕业论文关键词：灵芝；大孔树脂；高效液相色谱；细胞毒性；三萜化合物
The active ingredients in Ganoderma lucidum extraction and enrichment technology research
Abstract: Ganoderma Lucidum Karst was also known as Ganoderma lucidum， ganoderma,Jun Lingzhi, and lucid ganoderma, which has the efficacy of relieving cough and asthma, conserving vitality, and soothing the nerves. It treats the diseases like consumptive cough, dizzy insomnia, palpitations, and shortness of breath. The chemical composition of G. lucidum is complex. Its chemical composition is different by the species, culture method, and extraction process. The research shows that the G. lucidum has triterpenoids and polysaccharide compounds besides sterols, nucleoside, alkaloids, furan derivatives, amino acid polypeptide, inorganic elements and fatty acids, etc., but triterpenoids and polysaccharides were the main active substance in G. lucidum. The purpose of the research is not only to understand the chemical composition difference between the different and same species of the G.lucidum in different development phase, but also to make sure the active ingredient and content of the G. lucidum by extraction method.
The study was through the Pilot Extraction of the Hunong number 1 G.lucidum in fifty kilogram prior stage. Then take parts of the extract with 50%, 60%, 70%, 80%, 90%, and 100% ethanol solution into D-101, HZ-801, and HZ-818 macroporous resin respectively. The discovery of the elution experiments was that the most suitable macroporous resin to enrich the G.Lucidum triterpenoid was D-101 macroporous resin. And then use each segment eluting extract through High Performance Liquid Chromatography analysis and antitumor activity test. The result of analysis showed that the triterpenoid mainly in 90% and 100% alcohol extract of G.lucidum liquid, and the eluate of this part had strong cytotoxic activity. The following experiment was to elute macroporous resin, which finally obtained the component filled with triterpenoid. And in the end take the component into silica gel column chromatography, which achieved two compounds ergosterol and ganodermanontriol.
Keywords: Ganoderma Lucidum； macroporous resin；High Performance Liquid Chromatography； cytotoxicity； triterpenoid
目录
1 前言 1
1.1 灵芝概况 1
1.2 灵芝三萜的药理活性研究概况 2
1.2.1抗肿瘤作用 2
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