基于FISH的蚕豆染色体核型分析
毕业论文关键词：蚕豆；寡核苷酸探针；多色-顺序ND-FISH；核型
Karyotype analysis of Faba bean chromosomes based on FISH
Abstract: Faba bean is an important edible legume crop in China, its seeds are nutritious and have high-protein and low-fat which has a large research and application potential. However, the cytological markers in this species are very few and not effective for distinction and identification of the same species of different chromosome or arms. In this paper, the oligonucleotide probes developed by Nanjing Agricultural University were analyzed and the major results indicated that twelve oligonucleotide probes produced stable and clear signals which included（AAT）19,（ATT）10,（ATT）19,（ACC）19, (AGG)10,（GAA）10,（GA）29,（AT）29, 18s-1, AFA-4, pSc119.2-1A-2 and TEL–FF. By comparison, we found that (AAT)19, (ATT)19, (ATT)10, (AGG)10, AFA-4 and pSc119.2-1A-2 produced similar signals in the middle and proximal centromeric region of most chromosomes, while (ACC)19 and (AT)29 produced signals in the middle, proximal region and the ends of chromosomes, TEL-FF not only distributed across all chromosome ends, but also has strong amplification signals at the proximal telomeres of two pairs of chromosome short arms and has weak but clear hybrid signals in the proximal centromeres of all chromosomes. Based on the distribution of AFA-4 and TEL-FF, the karyotype of the broad bean was developed which could accurately identify all the chromosomes of broad beans and provides a simple, economical and new method for broad bean chromosomes engineering.
Key words: faba bean; oligonucleotides; multicolor-sequential ND- FISH; karyotype
目 录
摘要3
关键词3
Abstract3
Key words4
引言4
1材料与方法5
1.1材料 6
1.1.1植物材料6
1.1.2探针6
1.2方法7
1. 2. 1材料处理与取材7
1 .2. 2根尖的固定7
1.2.3根尖细胞有丝分裂中期染色体制片7
1.2.4非变性FISH7
1.2.5染色体分析7
2结果与分析7
2. 1蚕豆根尖细胞染色体制片方法7
2. 2寡聚核苷酸探针的筛选8
2.3蚕豆核型的建立.11
3讨论.12
致谢13
参考文献13
基于FISH的蚕豆染色体核型分析
引言：
蚕豆（Vicia faba L.）是我国重要的食用豆科作物。蚕豆籽粒营养丰富，属高蛋白低脂肪作物，是一种重要的植物蛋白资源。近年来，国内外学者们对蚕豆的营养特点、种质资源、品种改良进行了大量研究。但是,研究发现，由于目前栽培利用的蚕豆品种单一、遗传基础较狭窄、育种方法单一、基础研究较为薄弱，所以蚕豆遗传改良工作尚具有很大的发展空间[1]。