不同氮素水平对盐胁迫下菘蓝种子萌发和幼苗生长的影响
毕业论文关键词： 菘蓝；氮素水平；盐胁迫
Effects of different nitrogen levels on seed germination and seedling growth of Isatis indigotica under salt stress
Abstract: Objection: In order to provide scientific basis for the cultivation of Isatis indigotica on saline land and the full use of nitrogen, the effects of different nitrogen levels on seed germination and seeding growth of Isatis indigotica from 2 different origins under salt stress were studied. Method: With the I.indigotica from Hebei Baoding (HB) and Shandong Binzhou (SB) as research materials, the changes of seed germination, seedling growth and the physiological and biochemical characteristics of I.indigotica after 5 different nitrogen levels treatment（T1：3.75 mmol•L-1、T2：7.5 mmol•L-1、T3：15 mmol•L-1、T4：18.75 mmol•L-1、T5：22.5 mmol•L-1）under salt stress of NaCl (100 mmol•L-1 ) were studied, and the 15 mmolL-1 nitrogen treatment without salt was used as control (CK). Result: Salt stress inhibited seed germination and seedling growth of I.indigotica from 2 origins under normal nitrogen levels, and induced the increase of SOD activity, MDA , proline and soluble protein content in I.indigotica seedlings. The seeds germination rate, seedling biomass and contents of osmotic adjustment substances of Isatis indigotica from 2 origins are lower than other nitrogen levels under low nitrogen (T1, T2) treatment under salt stress. Besides, the effects of high nitrogen treatment (T4, T5) on the above indexes were different, too high level of nitrogen treatment (T5) will lead to the decrease of seeds germination rate, seedling biomass, and the content of osmotic substances of Isatis indigotica from 2 origins. Conclusion: High nitrogen level of T4
treatment could significantly promote seed germination and seedling growth of I.indigotica from 2 origins under salt stress, and improve the adaptability of I.indigotica seedlings from 2 origins under salt stress.
Key words: Isatis indigotica; Nitrogen level; Salt stress
目录
摘要1
关键词1
Abstract1
Key words2
引言2
1 材料与方法2
1.1 材料 2
1.2 试验方法 2
1.3 测定指标及方法3
1.3.1 种子发芽指标的测定3
1.3.2 幼苗生长指标和生物量的测定3
1.3.3 超氧化物歧化酶（SOD）活性的测定3
1.3.4 丙二醛（MDA）含量的测定3
1.3.5 可溶性蛋白含量的测定3
1.3.6 脯氨酸含量的测定 3
1.4 数据分析3
2 结果与分析3