油菜的F-BOX蛋白序列特征与生物信息分析
毕业论文关键词：油菜；F-BOX蛋白；生物信息学
Sequence characters and Bioinformatics Analysis of F-BOX Protein in a Brassica napus L
Abstract:F-BOX protein is a kind of protein family with F-BOX structure domain widely existing in eukaryotic organism,which has the function of the substrate recognition characteristics in the process of the protein ubiquitin mediated hydrolysis, and plays an important role in apoptosis,cell cycle regulation, cell signal transduction, transcription regulation and so on.Downloaded from the NCBI database F-BOX protein sequence of Brassica napus L, using bioinformatics technology, the expression of the basic physical and chemical properties, electronic, gene structure and conservative, disorder of protein domain area and globulin area, secondary structure and tertiary structure is analyzed and forecasted.The results showed that the content of 20 kinds of amino acids in F-BOX protein sequence of Brassica napus L is unbalanced;The protein is spherical hydrophilic protein, no transmembrane region, excluding signal peptide protein N end and is not a secreted protein;The protein gene have five exons, which is mainly expressed in flowers and seeds;Secondary structure is given priority to with random curl;The protein is the alpha protein with Swiss-Model for modeling,and Ramachandran map test showed that the Model structure comply with the rules of stereochemistry.This paper provides a theoretical basis for further studying the F-BOX protein structure and function .
Key Words: Brassica napus L; F-BOX protein; Bioinformatics
目 录
摘 要 1
引言 2
1. 材料和方法 2
1.1材料来源 3
1.2 理化性质分析，紊乱区和球蛋白区预测，跨膜区分析 3
1.3 电子表达、保守域、信号肽分析 5
1.4 磷酸化、亚细胞定位、基因结构分析 7
1.5 二级结构和三级结构预测，对三级结构预测结果的评价 8
1.6 同源比较和进化分析 11
2. 结果与分析 12
2.1 油菜的F-BOX蛋白序列的理化性质分析 13
2.2 油菜的F-BOX蛋白序列的紊乱区和球蛋白区预测，跨膜区分析 14
2.3 油菜的F-BOX蛋白序列的磷酸化、亚细胞定位和基因结构分析 17
2.4 油菜的F-BOX蛋白序列的电子表达、保守域和信号肽分析 18
2.5 二级结构和三级结构预测，对三级结构预测结果的评价 19
2.5.1 二级结构预测 19
2.5.2 三级结构预测，对三级结构预测结果的评价 19
2.6 垂直同源序列比较和进化分析 20
2.6.1 油菜的F-BOX蛋白垂直同源序列比较 20