鲫鱼皮肤维隆气单胞菌的分离鉴定与致病性评价
毕业论文关键词：鲫鱼；文隆气单胞菌；分离；生理生化；药敏试验；感染
Isolation、Identification and Pathogenieity Evaluation of Pathogenic Bacteria Aeromonas veronii from skin of Carassius aumtus
Abstract: The bacteria isolated from pathogenic skin of diseased crucian carp (Carassius aumtus) is temporarily designated for the KP. And we purified the bacteria with the TSA medium separation. Then, we conducted the morphology observation through Gram staining, the test of physiological and biochemical characteristics, the test of drug sensitive, and the artificial infection. The results showed that the KP strains is short of gram-negative bacillus; The results of the physiological and biochemical tset showed that the KP strains are all emerged the positive reaction to the glucose producing gas, MR, VP, urease, phenylalanine, lysine and ornithine, sucrose, O/F biochemical tube, double hydrolytic enzymes, amino acids contrast; And the KP strains are all emerged the negative reaction to the hydrogen sulfide, citric acid, salt, glucose acid salt, indole, raffinose, sorbitol, side marigold alcohol, the alcohol, lactose, evonymun alatus alcohol, arabinose, seven leaf glycosides, inositol, xylose and rhamnose, DNA enzyme. According to the Common Bacteria System Identification Manual, we identified the bacteria as Aeromonas veronii preliminarily. The test results of the drug susceptibility showed that the strain of KP is highly sensitive to the carbon penicillium alkene, cephalosporins, aminoglycoside, quinolone, sulfa large ring lactone class; And the strain of KP is moderately sensitive to the piperacillin, vancomycin, rifampin; And the strain of KP is low-alcohol sensitive to the penicillin class, clindamycin. The results of artificial infection to crucian carp showed that the fatality rate of the KP strains was 100% among 72 h, and the carp emerged the symptoms of lousy tail, ulcer, skin hyperemia bleeding ect. This experiment will provide certain theoretical basis for bacterial identification and the prevention of fish diseases .
Key words:Crucian carp; Velon Aeromonas; Isolation; Physiological and
Biochemical ; Drug sensitivity test; Infection.
目录
引言： 1
1材料与方法 2
1.1 实验动物 2
1.2 实验主要试剂及仪器 2
1.3 实验方法 2
1.3.1 细菌的分离纯化 2
1.3.2 菌落形态观察、生理生化特性 2
1.3.3 药敏实验 3
1.3.4 人工感染试验 3
2 结果与分析 3