外源亚精胺对玉米叶片抗涝性影响的研究
毕业论文关键词：亚精胺；涝害胁迫；POD；CAT；MDA；电导率；叶绿素
Study on The Effects of Exogenous spermidine on The Resistance of Maize Leaves Under Waterlogging Stress
Ping Yanfei Supervisor: ZHANG Jian
(School of Life sciences,Jiangsu Normal University,Xuzhou,Jiangsu,221116,China)
Abstract:Maize (Zea mays L.) ,one of the grain crops in China, was very important on agricultural production, but waterlogging was an important abiotic stress factors of its growth and yield.Therefore, seeking for effective methods to improve waterlogging resistance of maize was one of the most important aspects of stress resistance in maize.This experiment adopted Nongda 95 as materials, the effect of spraying pretreatment of maize leaves growth and physiological characteristics researching.The results showed that exogenous spermidine pretreatment can enhance the waterlogging resistance of maize leaves,and its specific performance for the obvious enhancement of peroxidase (POD), catalase (CAT) activity, alleviated the fall of the content of chlorophyll,while alleviating the increase of malondialdehyde (MDA) caused by the peroxidation of membrane and the increase of conductivity caused by the electrolyte leakage.
Key words :Spermidine; Waterlogging stress; POD;CAT; MDA; Conductivity
目录
引言 1
1 材料与方法 2
1.1 材料 2
1.1.1 供试材料 2
1.1.2 供试材料的培养与处理 2
1.1.3 试剂 2
1.1.4 设备 3
1.2 测定项目与方法 3
1.2.1 酶液的提取 3
1.2.2 过氧化物酶活性测定 3
1.2.3 过氧化氢酶活性测定 3
1.2.4 丙二醛含量测定 4
1.2.5 电导率大小测定 4
1.2.6 叶绿素含量测定 4
2 结果与分析 4
2.1 亚精胺对涝害胁迫下玉米叶片过氧化物酶活性的影响 5
2.2 亚精胺对涝害胁迫下玉米叶片过氧化氢酶活性的影响 5
2.3 亚精胺对涝害胁迫下玉米叶片丙二醛含量的影响 6
2.4 亚精胺对涝害胁迫下玉米叶片电导率的影响 7
2.5 亚精胺对涝害胁迫下玉米叶片叶绿素含量的影响 8
3 讨论 8
致谢 9
参考文献 10
引言
植物生长发育需要很多内在外在的条件，而水分就是其中很重要的一点，水分不足或过多对植物生长都是不利的。自20世纪80年代以来，人类的活动空间不断扩大，改造自然的能力也在增强，破坏森林、围垦湖泊等行为造成水土流失，容易引发涝害[1]。研究表明，洪涝对植物生长发育危害较大是因为水分过多会引起根部缺氧，进而影响地上部分，造成植物代谢紊乱、乙烯增加、营养失调等一系列的影响[2]。在农业生产方面，这种效应会造成农作物减产，严重了会没有收成，阻碍农业进步的步伐。
下一篇：花马池盐湖嗜盐细菌多样性研究