致病菌RXLR效应蛋白的序列特征与功能位点预测
毕业论文关键词：效应蛋白; 致病疫霉菌; RXLR基序; 序列特征; 功能位点
Pathogenic Bacteria RXLR Effector Protein Sequence Characteristics and Function of Site Prediction
Abstract：Pathogen effectors are small secreted proteins delivered by parasites to either the apoplastic space or the cytoplasm of plant cells. Plant pathogenic bacteria, fungi and oomycetes all use effector proteins during infection .During Plants and pathogen against each other in the process, some pathogenic bacteria secrete proteins into the host cell. These proteins known as protein (AVR) effector, can restrain plant immune response through a variety of ways, to help the pathogen infect plants successfully. In this study, we select RXLR effector proteins of Pathogens as the research object , using bioinformatics methods to analyze the phylogenetic relationship of RXLR effect protein, conservative motif, function structure domain, and the projections for its functional sites. The results showed that 106 species of protein RXLR effect were pided into 7 groups (Avr1a group, Avr1b group, Avr2 group, Avr3a group, Avr3b group, Avrblb1 group, Avrblb2 group). All of the 106 kinds of effector proteins contain RXLR conservative motif, some of which posseses other structural domain besides RXLR base sequence. The conservatism of the protein RXLR effect is obvious.
Key words: Effect of protein, Phytophthora infestans, RXLR motif, Characteristics of Sequence, Functional sites
目 录
摘要 1
引言 2
1 数据与方法 4
1.1 数据来源与序列检索 4
1.2 序列比对与聚类分析 4
1.3 RXLR蛋白保守基序分析 4
2 结果与分析 4
2.1致病菌RXLR效应蛋白序列的鉴定 4
2.2 致病菌RXLR效应蛋白基因的系统进化关系 5
2.3 致病菌RXLR效应蛋白保守基序组成模式 6
2.4致病菌RXLR效应蛋白功能结构域 8
2.5 致病菌RXLR效应蛋白的功能位点预测 8
3 讨论与结论 10
3.1进化树与保守基序模式 10
3.2效应蛋白功能结构域与功能位点预测 10
4展望 11
参考文献 12
致谢 13
致病菌RXLR效应蛋白的序列特征与功能位点预测
引言
研究表明，效应蛋白不仅能在生理、代谢机制上控制植物气孔开闭、抑制胼胝质沉积、抑制ROS产生以及HR超敏反应，还在分子、生化机制上调控寄主细胞相关基因表达、 分子模拟调控各种信号通路、阻断 MAPKs 通路等[1-3]。效应蛋白在被植物识别的情况下也被称为无毒蛋白,它是植物病原菌在与寄主植物相互作用过程中分泌的、可被植物(NBS-LRR)抗性蛋白特异识别、兼具毒性与无毒功能的蛋白质分子。根据对植物NBS-LRR抗性蛋白的功能分析及其在植物细胞中的定位,目前认为细胞质是效应物蛋白与抗性蛋白直接或间接结合互作的场所。病原菌效应蛋白是具有多结构域、多功能的分泌蛋白，在病原菌与植物互作过程中扮演着不可忽视的重要的角色。
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