基于16SrRNA基因序列甲拌磷污染底泥细菌多样性研究
毕业论文关键词：甲拌磷；细菌；454焦磷酸测序；生物多样性。
The bacterial persity in phorate contaminated sediment based on 16S rRNA gene sequences
YU Hong Supervisor: WEN Hongyu
(School of Life Sciences, Jiangsu Normal University, Xuzhou, Jiangsu, 221116, China)
Abstract:【Object】The aim of the study was to evaluate the bacterial persity in the contaminated soil by the sewage released by a pesticide factory( mainly produced phorate). Two soils near the pesticide factory devoted in the past to sewage discharging activities were selected: one(NYC) situated near the sewage outlet and the other one(NYCDN) placed in approximately 2 km away from the sewage outlet. We will compare the bacterial persity in two sites(NYC, NYCDN). It helps to understand the interaction between the environmental pollution and ecological change.【Methods】The persity of bacterial communities were assessed by the culture-independent methods (the 454 pyrosequencing technology). 【Results】The Chao1, ACE richness estimator, the Shannon and Simpson persity indices were used to estimate the bacterial species richness at a 3% dissimilarity level in each sample. There're a total of 1023 and 1031 OTU in the NYC and NYCDN, respectively.A total of 80 genera in NYC were detected by pyrosequencing, while there were almost there were almost twice as many genera in NYCDN.Furthermore, the most predominant bacterial phyla in NYC was Proteobacteria (51.89%)and Firmicutes(44%), respectively. Besides, the most predominant bacterial phyla in NYCDN was Firmicutes(44%) and Proteobacteria(35.58%), respectively. In addition, the most prevalent genera observed were Urania-1B-19 marine sediment group(Phycisphaerae class, Planctomycetes phylum) in NYC and Ruminococcaceae (Bacilli class, Firmicutes phylum)in NYCDN among all the 199 different bacterial genera, respectively.【Conclusion】It was possible to conclude that bacterial species richness in NYC was significantly lower than that of bacteria in NYCDN. With the flowing of the phorate sewage from NYC to NYDCN, soil bacterial persity was increased in NYDCN. This may be related to the higher phorate concentrations in NYC.The results showed that sewage from the pesticide factory had been seriously affected the persity of soil bacteria. It also showed the positive influence of the soil bacteria , which means there existed certain bacteria that have the ability to deal with the phorate pollutants.
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