铜绿微囊藻培养体系对抗生素的降解研究
毕业论文关键词：铜绿微囊藻、抗生素、降解
Study on degradation of antibiotics by cultivation system of Microcystis aeruginosa
Abstract：Microcystis aeruginosa belongs to Cyanophyta chroococcaceae Microcystis, which is one of the dominant algae bloom.Because of the proliferation of cyanobacteria, common in the eutrophication of the water body, so as to the eye to form a water bloom.Among them, two kinds of substances, such as the aeruginosa and the antibiotic, may exist simultaneously.Therefore, the study is on the degradation of antibiotics in the culture system of aeruginosa and the Evaluation of the environmental toxicity of antibiotics and the risk of environmental impact.Let us further understand the characteristics of the two types of substances such as aeruginosa and antibiotics And the rational use of antibiotics and on how to correctly evaluate the ecological safety play an important significance. High performance liquid chromatography was used for the detection of the degradation of antibiotics in the liquid of P. aeruginosa.And the content of chlorophyll of the P. aeruginosa was determined by UV spectrophotometer.In this study, the degradation of antibiotics in Pseudomonas aeruginosa was studied.
Keyword:Microcystis aeruginosa antibiotic degradation
目录
1 绪论 7
1.1 铜绿微囊藻 7
1.1.1 铜绿微囊藻的特征 8
1.1.2 铜绿微囊藻的生态学概述 8
1.1.3铜绿微囊藻的生态学现状 9
1.2 抗生素 10
1.2.1 抗生素介绍 12
1.2.2 环境抗生素的来源 12
1.2.3 环境抗生素的危害 13
1.2.4 环境中抗生素的残留 14
2 实验条件及预实验 14
2.1 实验条件 14
2.2 实验药品、器材 15
2.3 预实验 15
2.3.1 高压蒸汽灭菌 15
2.3.2 铜绿微囊藻的接种 16
2.3.3 铜绿微囊藻的条件 16
3. 实验方案与方法 16
3.1 四环素类抗生素储备液的配置 16
3.2高效液相色谱仪检测铜绿微囊藻培养体系对抗生素的降解 17
3.2.1实验原理 17
3.2.2HPLC的特点与优点 17
3.2.3流动相的选取 18
3.2.4实验方法 18
4. 实验结果与讨论 18
4.1高效液相色谱仪检测四环素类抗生素在铜绿微囊藻液中的降解 20
4.1.1标准曲线 20
4.1.2降解曲线 20
5 结论 20
1 绪论
1.1 铜绿微囊藻
1.1.1铜绿微囊藻的特征
铜绿微囊藻是现今较为普遍的水华蓝藻，它是一种原核生物。藻细胞的体积非常的小，它的直径大小仅为3-7毫米。而细胞的外部是由鞘包覆。它的细胞内有一种呈现蓝绿色的原生质体。但是在光学显微镜里面观察铜绿微囊藻是呈现暗色的。这是因为该藻细胞的囊胞充满了空气。而存在于细胞里的囊胞的作用是提供足够的浮力。这可以使藻细胞获得更好的光线与二氧化碳。
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