摘要本次实验主要采集了徐淮山羊胎羊的8种组织，即心、肝、脾、肺、肾、肠、背肌和腿肌，然后采用实时荧光定量PCR的方法，研究分析这8种组织中ZFAND5基因的表达差异性。首先是从徐淮山羊胎羊的8种组织中提取出总RNA，其次反转录成cDNA，再运用real-time PCR技术在各组织中检测ZFAND5基因的表达水平。通过实验得到了在徐淮山羊胎羊的8种组织中ZFAND5基因表达水平依次为心肌（1.0000）、腿肌（0.7318）、背肌（0.1467）、肺(0.0559)、肝(0.0325)、肾(0.0215)、脾(0.0095)、肠(0.0091)。由此可见，ZFAND5基因在徐淮山羊胎羊的肌肉组织中呈高水平的表达，而在其他组织表达量较低，可以推测ZFAND5基因可能与徐淮山羊胎羊的肌肉组织的生长发育和功能有关系。此外，本次实验所得的ZFAND5基因在徐淮山羊胎羊的8种组织中的表达情况，也可为今后研究山羊的ZFAND5基因提供参考。42694

该毕业论文有图5幅，表5个，参考文献13篇。

关键词：徐淮山羊  ZFAND5基因  实时荧光定量PCR  差异性表达 

The Study on Difference Expression of ZFAND5 Gene in Xuhuai Fetal Goat Organs

Abstract

The experiments were collected from the eight organizations of Xuhuai fetal goat, namely heart, liver, spleen, lung, kidney, intestine, back muscle and leg muscle, and apply real-time PCR method to study the difference expression of ZFAND5 gene in the eight organizations. The first is extracted the total RNA from the eight organizations of Xuhuai fetal goat , followed by reverse transcribed into cDNA, then using real-time PCR technology to detect the expression levels of ZFAND5 gene in tissues. Through the experiments, the expression levels of ZFAND5 gene in the eight organizations of Xuhuai fetal goat were myocardium (1.0000), leg muscle (0.7318), back muscle (0.1467), lung (0.0559), liver (0.0325), kidney ( 0.0215), spleen (0.0095), intestine (0.0091). Thus, high levels of ZFAND5 gene was expressed in the muscle tissue of Xuhuai fetal goat,while lower expression in other tissues,presumably ZFAND5 gene may be associated with the growth and development and the function of muscle tissue in Xuhuai fetal goat. In addition, the results of this experiment about the difference expression of ZFAND5 gene in the eight organizations also can provide reference for future research on goats ZFAND5 gene.

Key Words: Xuhuai goat  ZFAND5 gene  real-time PCR  different expression

目  录

摘  要 I

Abstract II

目  录 III

图清单 IV

表清单 IV

1绪论 1

2 材料与方法 2

2.1材料 2

2.2实验方法 3

2.3统计方法 6

3 结果与分析 6

3.1 RNA质量检测结果 6

3.2 PCR定量检测结果 7

3.3 内参基因与ZFAND5基因的溶解曲线与扩增曲线 8

3.4 ZFAND5基因差异性表达结果 9

4讨论 10

5 结论 12

参考文献 13

致谢 14

图清单

图序号 图名称 页码

图3-1